Halohydrin dehalogenases (HHDHs) are promising enzymes for application in biocatalysis due to their promiscuous epoxide ring-opening activity with various anionic nucleophiles. So far, seven different HHDH subtypes A G have been reported subtype D containing the by far largest number of enzymes. Moreover, several characterized members display outstanding characteristics such as high catalytic a...

The membrane-bound tetrachloroethene reductive dehalogenase (PCE-RDase) (PceA; EC 1.97.1.8), the terminal component of the respiratory chain of Dehalobacter restrictus, was purified 25-fold to apparent electrophoretic homogeneity. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a single band with an apparent molecular mass of 60 +/- 1 kDa, whereas the native molecular mass wa...

Title of Document: THE ROLE OF DEHALORESPIRING BACTERIA IN THE REDUCTIVE DECHLORINATION OF POLYCHLORINATED BIPHENYLS IN BALTIMORE HARBOR SEDIMENT MICROCOSMS Sonja Kristine Fagervold, Doctor of Philosophy, 2007 Directed By: Professor Kevin R. Sowers, Marine Estuarine Environmental Sciences Baltimore Harbor sediment microcosms were incubated with the 12 most predominant congeners in Aroclor 1260 ...

The haloalkane dehalogenase DhaA from Rhodococcus rhodochrous NCIMB 13064 is a bacterial enzyme that shows catalytic activity for the hydrolytic degradation of the highly toxic industrial pollutant 1,2,3-trichloropropane (TCP). Mutagenesis focused on the access tunnels of DhaA produced protein variants with significantly improved activity towards TCP. Three mutants of DhaA named DhaA04 (C176Y),...

4-chlorobenzoyl-coenzyme A (4-CBA-CoA) dehalogenase catalyzes the hydrolytic dehalogenation of 4-CBA-CoA by attack of Asp145 on the C4 of the substrate benzoyl ring to form a Meisenheimer intermediate (EMc), followed by expulsion of chloride ion to form an arylated enzyme intermediate (EAr) and, finally, ester hydrolysis in EAr to form 4-hydroxybenzoyl-CoA (4-HBA-CoA). This study examines the c...

Reductive dehalogenases are essential enzymes in organohalide respiration and consist of a catalytic subunit A and a membrane protein B, encoded by rdhAB genes. Thirty-two rdhAB genes exist in the genome of Dehalococcoides mccartyi strain CBDB1. To gain a first insight into the regulation of rdh operons, the control of gene expression of two rdhAB genes (cbdbA1453/cbdbA1452 and cbdbA1455/cbdbA1...

Desulfitobacterium spp. Gábor, Krisztina − Molecular analysis of halorespiration in Desulfitobacterium spp.: catalysis and transcriptional regulation Szüleimnek Gábor, K. (2006). Molecular analysis of halorespiration in Desulfitobacterium spp.: catalysis and transcriptional regulation. PhD thesis. Soil and ground water contamination by halogenated organic compounds mainly used as biocides in ag...

We used directed evolution to obtain enantiocomplementary haloalkane dehalogenase variants that convert the toxic waste compound 1,2,3-trichloropropane (TCP) into highly enantioenriched (R)- or (S)-2,3-dichloropropan-1-ol, which can easily be converted into optically active epichlorohydrins-attractive intermediates for the synthesis of enantiopure fine chemicals. A dehalogenase with improved ca...