It is hypothesized that membrane-associated iron in the sickle red cell is of pathophysiologic importance, but the actual existence of such iron in the intact cell has been questioned. Using a strategy whereby membrane iron can be detected through its bioavailability for catalyzing peroxidation, we used phospholipid exchange protein to load membranes of intact erythrocytes (RBC) with approximat...

BACKGROUND The duration of storage of transfused red blood cells (RBC) has been associated with poor clinical outcomes in some studies. We sought to establish whether prolonged storage of transfused RBC in cancer patients influences overall survival (OS) or cancer recurrence. METHODS AND FINDINGS Patients diagnosed with cancer at The Ottawa Regional Cancer Centre between January 01, 2000 and ...

In a number of clinical circumstances it would be desirable to artificially conceal cellular antigenic determinants to permit survival of heterologous donor cells. A case in point is the problem encountered in transfusions of patients with rare blood types or chronically transfused patients who become allosensitized to minor blood group determinants. We have tested the possibility that chemical...

Human erythrocytes (RBC) from whole blood were separated according to their specific densities by centrifugation on a polyvinyl-pyrrolidine-coated colloidal silica matrix (Percoll) into four major subpopulations. By indirect immunofluorescence assay, the most dense RBC subpopulation, with specific density greater than 1.110 g/ml (3%-5% of total RBC), was positive for membrane-bound immunoglobul...

INTRODUCTION Red blood cells' (RBC) rheological properties are disturbed in chronic venous disease (CVD). The aim of the study was to compare deformability and aggregation of erythrocytes taken from the varicose vein and the antecubital vein of patients with chronic venous disease. MATERIALS AND METHODS Blood samples were taken from twelve CVD patients presenting clinical, aetiological, anato...

acid, or both and after cessation of such supplementation, which would, by necessity, involve dynamics between RBC kinetics and whole-body folate kinetics. Quinlivan further comments that a steady state of RBC folate may have been reached between weeks 16 and 24. In our intervention study, blood drawings were performed every 4 wk, and RBC folate significantly increased between week 16 and week ...