Target substrate-specific hammerhead ribozyme cleaves the specific mRNA efficiently and results in the inhibition of gene expression. In humans, overproduction of apolipoprotein B (apoB) is positively associated with premature coronary artery diseases. The goal of this study is to demonstrate that long-term reduction of apoB gene expression using hammerhead ribozyme would result in inhibition o...

Nonalcoholic fatty liver disease (NAFLD) is a major contributing factor to hepatic insulin resistance in type 2 diabetes. Diacylglycerol acyltransferase (Dgat), of which there are two isoforms (Dgat1 and Dgat2), catalyzes the final step in triglyceride synthesis. We evaluated the metabolic impact of pharmacological reduction of DGAT1 and -2 expression in liver and fat using antisense oligonucle...

This experiment was aimed to study the association between the DGAT1 K232A polymorphism and milk production traits and somatic cell score (SCS) in Iranian Holstein dairy cows. The records of 408 animals from five dairy herds were randomly identified and then genomic DNA was extracted from blood using the modified-salting method described by Miller. RFLP-PCR was performed to obtain all the polym...

KADLECOVA, V., D. NEMECKOVA, K. JECMINKOVA and L. STADNIK, 2014. The effects of polymorphism in the DgAt1 gene on energy balance and milk production traits in primiparous Holstein cows during the first six months of lactation. Bulg. J. Agric. Sci., 20: 203-209 The aim of this study was to analyze the effects and significance of DGAT1 K232A polymorphism on milk production traits – milk yield, co...

BACKGROUND Jatropha curcas is an important biofuel crop due to the presence of high amount of oil in its seeds suitable for biodiesel production. Triacylglycerols (TAGs) are the most abundant form of storage oil in plants. Diacylglycerol O-acyltransferase (DGAT1) enzyme is responsible for the last and only committed step in seed TAG biosynthesis. Direct upregulation of TAG biosynthesis in seeds...

Mammary gland development is a complex process that is dependent on interactions between the developing mammary epithelium and the surrounding stromal tissues. We show that mice lacking the triglyceride synthesis enzyme acyl CoA:diacylglycerol transferase 1 (DGAT1) have impaired mammary gland development, characterized by decreased epithelial proliferation and alveolar development, and reduced ...

This experiment was aimed to study the association between the DGAT1 K232A polymorphism and milk production traits and somatic cell score (SCS) in Iranian Holstein dairy cows. The records of 408 animals from five dairy herds were randomly identified and then genomic DNA was extracted from blood using the modified-salting method described by Miller. RFLP-PCR was performed to obtain all the polym...