Human embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of in vitro fertilized human blastocysts. We examined the potential of eight growth factors [basic fibroblast growth factor (bFGF), transforming growth factor beta1 (TGF-beta1), activin-A, bone morphogenic protein 4 (BMP-4), hepatocyte growth factor (HGF), epidermal growth factor (EGF), beta nerve growth fact...

background: the mechanism of cadmium induced osteoporosis is not well understood, so in this study, we examined the toxicity of bone marrow mesenchymal stem cell (mscs) following treatment of rats with cdcl2 in drinking water, to revile the effect of this chemical on differentiation potential of mscs. methods: at the end of third passage, mscs were grown in the osteogenic medium for 21 days. to...

in this present study, we examined the differentiation potential of human bone marrow derived mesenchymal stem cells (hbmscs) into hepatocytes on a three-dimentional (3d) nanofibrous scaffold formed by poly (ε-caprolactone) (pcl), collagen and polyethersulfone (pes). the nanofiber was prepared by the electrospining technique. hbmscs were isolated using combining gradient density centrifugation ...

background: human embryonic stem cells (hesc), which are derived from the inner cell mass of the blastocysts, have been considered to be pluripotent cells. in this study we examine the differentiating potential of hesc into hepatocytes by characterization of the expression of endoderm and liver-specific genes. methods: hesc were cultivated in suspension to form aggregates, the embryoid bodies. ...

new blood forming vessels are produced by differentiation of mesodermal precursor cells to angioblasts that become endothelial cells (ecs) which in turn give rise to primitive capillary network. human cord blood (hcb) contains large subsets of mononuclear cells (mncs) that can be differentiated into endothelial-like cells in vitro. human mononuclear progenitor cells were purified from fresh umb...

Background: Vascular endothelial growth factor-A (VEGF-A), an endothelial cell-specific mitogen produced by various cell types, plays important roles in cell differentiation and proliferation. In this study we investigated the effect of recombinant VEGF-A on differentiation of mesenchymal stem cells (MSCs) to endothelial cells (ECs). Methods: VEGF-A was expressed in E. coli BL21 (DE3) and BL21...