DNA hybridization detection in microfluidic devices can reduce sample volumes, processing times, and can be integrated with other measurements. However, as device footprints decrease and their complexity increase, the signal-to-noise ratio in these systems also decreases and the sensitivity is thereby compromised. Device miniaturization produces distinct properties and phenomena with greater in...

We combine microfluidic system with digital lithography to achieve high throughput microparticle synthesis and labeling. Microfluidic system supports laminar flow, enabling multiple functional materials in one channel. By innovative application of a dynamic mask, we are able to fabricate microparticles from photocurable resin rapidly in the microfluidic channel. Microparticles with different fu...

Autoimmune bullous diseases (ABD) are a rarely seen group of diseases, of which pemphigus and bullous pemphigoid (BP) are the major groups. Diagnosis is generally based on the combination of clinical features, histopathologic and immunofluorescence (IF) findings, and/or enzyme-linked immunosorbent assay (ELISA). Aims of the work were to determine the value of the innovative BIOCHIP mosaic-based...

BACKGROUND Circulating biomarkers can predict clinical outcomes in colorectal cancer patients. The aim of the study was to evaluate the feasibility of our multigene biomarker chip for detecting circulating tumor cells for postoperative surveillance of stage I-III colorectal cancer patients. MATERIALS AND METHODS In total, 298 stage I-III colorectal cancer patients were analyzed after curative...

An oligonucleotide biochip that specifically detects point mutations in the gyrA and parC genes of Neisseria gonorrhoeae was designed and subsequently evaluated with 87 untreated clinical specimens. The susceptibilities of the N. gonorrhoeae strains were tested to determine the prevalence of ciprofloxacin-resistant strains in Anhui Province, People's Republic of China. Conventional DNA sequenci...

The aim of our research was to develop a numerical model microflows occurring in the culture chambers (CC) microfluidic device construction for high-throughput drug screening. incompressible fluid flow is based on lattice-Boltzmann equation, with an external body force term approximated by He-Shan-Doolen scheme and Bhatnagar-Gross-Krook approximation collision operator. accuracy validated algeb...

Silver-enhanced labeling method has been employed in immunochromatographic assays for improving the sensitivity of detecting pathogens. In this paper, we apply the silver enhancement technique for biomolecular signal amplification in a gold nanoparticle-based conductimetric biochip. We show that the response of the silver-enhanced biochip comprises two distinct regions namely: (a) a sub-thresho...

This paper presents an architecture for a portable system based on a magnetoresistive biochip microarray for DNA recognition or microorganism/cell identification. In this biochip, biological targets are labeled with magnetic nanoparticles which are used to guide target over surface immobilized probes. Furthermore, the magnetic stray field created by those labels is detected by on-chip magnetic ...

BACKGROUND Various antigen-specific immunoassays are available for the serological diagnosis of autoimmune bullous diseases. However, a spectrum of different tissue-based and monovalent antigen-specific assays is required to establish the diagnosis. BIOCHIP mosaics consisting of different antigen substrates allow polyvalent immunofluorescence (IF) tests and provide antibody profiles in a single...

This paper reports the first intensified biochip system for chemiluminescence detection and the feasibility of using this system for the analysis of biological warfare agents is demonstrated. An enzyme-linked immunosorbent assay targeting Bacillus globigii spores, a surrogate species for Bacillus anthracis, using a chemiluminescent alkaline phosphatase substrate is combined with a compact inten...