The present study demonstrates an efficient protocol for in vitro mass propagation of A. curassavica through direct as well as indirect regeneration. The effect of MS and L2 media supplemented with various concentrations and combinations of growth regulators has been studied. The growth regulators used include BAP and kinetin (cytokinins) and IAA, IBA, NAA and 2, 4-D (auxins). MS media proved b...

With the aim of micropropagation of Phyllanthus amarus, an important medicinal herb, shoot tips were cultured in Murashige and Skoog's medium supplemented with kinetin/ BAP singly or in combination with IAA. Growth regulators at lower range (0.1-1.0 mg L(-1)) stimulated direct regeneration of shoots. Kinetin was superior to BAP and kinetin-IAA combination was more suitable than kinetin alone. A...

parsi zira, bunium persicum (boiss.) b. fedtsch., or black zira, a member of the umbellifera family, is one of the most important medicinal species with high economic value. generally, there is limited information about in vitro culture of bunium persicum. in this study, the effect of various media (ms, b5) and combinations of plant growth regulators, naa and 2,4-d alone or together with kin, o...

In vitro studies were carried out with Ochradenus arabicus for rapid micropropagation from embryo explants. Direct regeneration of shoot buds of O. arabicus was observed in MS basal medium supplemented with varying levels of either benzyladenine (BA) or kinetin (Kn). Embryos produced multiple shoots on Murashige and Skoog’s medium (MS) containing 1.0 μM BA after 25 days of inoculation. Number o...

High frequency somatic embryogenesis and plant regeneration was achieved on calli developed from internodal and flower buds of E. foetidum. The explants were cultured on Murashige and Skoog medium supplemented with different concentrations and combinations of BA and IAA. The nodal explants produced multiple adventitious shoots while the internodal and flower buds induced callus, from which mult...