DNA adenine methylase mutants of Salmonella typhimurium contain reduced amounts of FinP, an antisense RNA encoded by the virulence plasmid pSLT. Lowered FinP levels are detected in both Dam- FinO+ and Dam- FinO- backgrounds, suggesting that Dam methylation regulates FinP production rather than FinP half-life. Reduced amounts of F-encoded FinP RNA are likewise found in Dam- mutants of Escherichi...

Seasonal subsurface temperature monitoring, which exploits the fact that increased seepage flow may locally alter distribution, is a useful approach for leakage monitoring and evaluation within embankment dams their foundations. At Mactaquac Generating Station, New Brunswick, Canada, spatial temporal variations of have been monitored modelled at steeply inclined interface between compacted clay...

In this study, we demonstrated that the methyltransferase activity associated with Dam was essential for attenuation of Aeromonas hydrophila virulence. We mutated aspartic acid and tyrosine residues to alanine within the conserved DPPY catalytic motif of Dam and transformed the pBAD/damD/A, pBAD/damY/A, and pBAD/damAhSSU (with the native dam gene) recombinant plasmids into the Escherichia coli ...

abstract one of the problems in design of irrigation structures is sedimentation control in the inlet to the irrigation networks. water quality for agriculture requires that the sediments be controlled and reduced to the permissible limit at their entrance point to the turnouts and irrigation networks. this is possible by constructing a sedimentation basin. the bigger the basin, the best the re...

a 57-year-old male with history of prostatectomy, cyctectomy, cecal urinary diversion for invasive prostate cancer and with a recent rise in psa level, was referred for bone scintigraphy to rule out osseous metastatic disease. an interesting finding was abnormal tracer accumulation throughout the large bowel, which was proved to be due to urinary diversion.

Several Emergency Medical Service (EMS) agencies across the US have demonstrated that the time spent by EDs on ambulance diversion can be reduced by implementing community-wide policies that restrict the duration and frequency of diversion episodes. However, the mechanisms through which these reductions materialize are not well understood. EDs can respond to such restrictions by improving their...

The DNA of Serratia marcescens has N6-adenine methylation in GATC sequences. Among 2-aminopurine-sensitive mutants isolated from S. marcescens Sr41, one was identified which lacked GATC methylation. The mutant showed up to 30-fold increased spontaneous mutability and enhanced mutability after treatment with 2-aminopurine, ethyl methanesulfonate, or UV light. The gene (dam) coding for the adenin...

The objective of this study was to evaluate the opinions and attitudes of dentists toward the use of rubber dam during various restorative procedures in their dental practices. A structured questionnaire was designed, and distributed to 300 dentists and those that were fully completed were collected for analysis. The collected informations about the opinions and attitudes of dentists toward the...

Urinary diversion after extirpative surgery of the bladder is done by various methods. Conduit urinary diversion is the most commonly practiced method of urinary diversion. It is relatively easy to perform and has a lower complication rate than other forms of diversion, e.g., orthotopic neobladder and continent cutaneous urinary diversion. Urolithiasis is a known and common complication of urin...