An analysis of the bichromophoric behavior and binding to the DNA base stack of the novel complex ruthenium 2,3-bis(phenylethynnyl)-I,4,8,-tetraazatriphenylene (Ru-bptt) and its tert-butyl substituted analogue (Ru-tbptt). This project attempts to revise the previously submitted manuscript of Structure, Spectroscopy and DNA Binding of Bichromophoric Mixed Ligand Ruthenium Metalloenedivne Complex...

Chemical studies have been carried out on the interaction of DNA with uranyl salts. The effect of variations in pH, salt concentration, and structural integrity of the DNA on the stoichiometry of the salt-substrate complex have been investigated. At pH 3.5 DNA interacts with uranyl ions in low concentration yielding a substrate metal ion complex with a UO(2) (++)/P mole ratio of about (1/2) and...

A novel tetranuclear [Pt4(Am4M)4]·16.25H2O (1), where H2Am4M is the thiosemicarbazone ligand (Z)-2-(amino(pyridin-2-yl)methylene)-N-methylhydrazinecarbothioamide, has been synthesized and characterized by using various physico-chemical techniques. X-ray analysis revealed that the Pt(II) complex consists of a neutral tetranuclear [Pt4(Am4M)4] unit and abundant water molecules. The tetranuclear u...

The peculiarities of binding the water-soluble meso-tetra-(4N-oxyethylpyridyl) porphyrin (TOEPyP4) with B-DNA and A-DNA have been studied by means of UV-VIS spectrophotometry and circular dichroism method. The binding constant (Kb) and stoichiometry (n) were determined based on the absorbance spectra for each DNA–porphyrin complex. The free energy, enthalpy and entropy of binding also were calc...

Abstract Ferrocene or ferrocenium has been widely studied in the field of organometallic complexes because its stable thermodynamic, kinetic and redox properties. Novel hexaferrocenium tri[hexa(isothiocyanato)iron(III)]trihydroxonium (HexaFc) complex was product from reaction ferrocene, maleic acid ammonium thiocyanate confirmed by elemental analysis CHNS, FTIR single crystal X-ray crystallogra...

Escherichia coli DNA photolyase is a DNA-repair enzyme that repairs cyclobutane pyrimidine dimers (CPDs) that are formed on DNA upon exposure of cells to ultraviolet light. The light-driven electron-transfer mechanism by which photolyase catalyzes the CPD monomerization after the enzyme-substrate complex has formed has been studied extensively. However, much less is understood about how photoly...

High-mobility-group proteins HMG-1 and HMG-I/Y bind to multiple sites within a 268 bp A/T-rich enhancer element of the pea plastocyanin gene ( PetE ). Within a 31 bp region of the enhancer, the binding site for HMG-1 overlaps with the binding site for HMG-I/Y. The kinetics of binding and the affinities of HMG-1 and HMG-I/Y for the 31 bp DNA were determined using surface plasmon resonance. Due t...

The initiation of transcription involves three steps: binding, opening, and escape of RNA polymerase. To model these steps in the simplest way we will treat opening and escape as a single reaction with forward reaction rate k determined by the regulatory proteins and their interaction with the DNA. Binding will be treated as a reversible reaction with an equilibrium constant KB. Using mathemati...

The characteristics of manganese and iron binding to human apotransferrin (apo-tf) have been investigated and compared in this study. Both metal ions were taken up by human apo-tf and formed complexes, with the maximum absorbances observed at 410 and 340 nm for manganese-transferrin (Mn-tf) and 465 nm for iron-transferrin (Fe-tf). Addition of manganese (1.5 µg/ml) to the reaction mixture contai...

The characteristics of manganese and iron binding to human apotransferrin (apo-tf) have been investigated and compared in this study. Both metal ions were taken up by human apo-tf and formed complexes, with the maximum absorbances observed at 410 and 340 nm for manganese-transferrin (Mn-tf) and 465 nm for iron-transferrin (Fe-tf). Addition of manganese (1.5 µg/ml) to the reaction mixture contai...