Fusarium solain is the most important pathogen of huge range of plant hosts, especially potato in the word, which causes tuber rot in storage and root rot of potato plants in fields. Fifty four isolates from potato, bean, chickpea and cucurbit (melon, watermelon and cucumber) was subjected in a study through analysis of vegetative compatibility groups (VCGs) and rep-PCR DNA fingerprinting. Nit ...

The DNA fingerprinting of fruit crops, based on microsatellite markers that are considered to be the key for molecular analysis germplasm collections, is reviewed. Simple sequence repeats (SSRs) remain choice in humans, animals, plants, and other living organisms. This review, considers 44 species, provides a set suitable profiling accessions should make databases produced different laboratorie...

Arbitrarily-primed DNA markers can be very useful for genetic fingerprinting and for facilitating positional cloning of genes. This class of technologies is particularly important for less studied species, for which genome sequence information is generally not known. The technologies include Randomly Amplified Polymorphic DNA (RAPD), DNA Amplification Fingerprinting (DAF), and Amplified Fragmen...

Phenotypic approaches based on metabolic and biological characteristics of Corynebacterium pseudotuberculosis have been limited due to insufficient discrimination between closely related isolates. In this paper we present performance and convenience of three molecular typing methods: BOX-PCR, random amplification of polymorphic DNA (RAPD) and amplification of DNA fragments surrounding rare rest...

We compared random amplified polymorphic DNA (RAPD) fingerprinting with cross-absorption agglutination and restriction enzyme analysis for typing bovine leptospires. Using RAPD fingerprinting, we examined a number of Leptospira serovars, namely, hardjo genotypes bovis and prajitno, pomona, balcanica, tarassovi, swajizak, kremastos, australis, and zanoni, which are likely to be isolated from Aus...

An easy and rapid protocol to extract DNA to be used as template for polymerase chain reaction (PCR) fingerprinting experiments from cultivable lactic acid bacteria (LAB) is proposed. Different procedures for rapid extraction of DNA by chelex (iminodiacetid acid) ionic resin were compared. Factors affecting the quality and reproducibility of PCR fingerprinting profiles were also investigated. T...