The efficient establishment of high protein producing recombinant mammalian cell lines is facilitated by the use of a stringent selection system. Here, we describe two methods to create a stringent selection system based on the Zeocin resistance marker. First, we cloned increasingly longer stretches of DNA, encoding a range of 8-131 amino acids immediately upstream of the Zeocin selection marke...

DNA metabarcoding is an increasingly popular technique to investigate biodiversity; however, many methodological unknowns remain, especially concerning the biases resulting from marker choice. Regions of cytochrome c oxidase subunit I (COI) and 18S rDNA (18S) genes are commonly employed “universal” markers for eukaryotes, but extent taxonomic introduced by these how such may impact performance ...

sex determination in pistacia as a dioecious plant is economically desirable. identification of the male and female plants has a great value for pistacia yield production. in the present study, leaf samples from four female plants from cultivars of pistacia including akbary, akbar aghaei, fandoghi, and kalleh ghochi as well as male plants were used for rapd pcr amplification. among 32 primers w...