Herpes simplex virus, grown in BHKzI cells, was extensively purified by fluorocarbon treatment, ultracentrifugation, sucrose gradient centrifugation and chromatography on calcium phosphate. The purity of the product was assessed by immunodiffusion, electrophoresis in polyacrylamide gels and removal of added radioactive host material from the virus. Purified virus was disrupted with sodium dodec...

Widely differing results were obtained for urinary protein determinations in male rats using 2 test strips and a quantitative Coomassie Blue sodium dodecyl sulphate method. A comparison of the sensitivity of the protein methods with respect to rat albumin and alpha 2U globulin was made; both test strips were found to be relatively insensitive to the presence of alpha 2U globulin. Resultant limi...

Large amounts of Cu-metallothionein were obtained by 2-mercaptoethanol and sodium dodecyl sulphate extractions of the particulate fractions of the liver of pigs given high-Cu2+ diets or rats injected with Cu2+. Three isoproteins were purified from pig liver and characterized on the basis of their physicochemical properties, metal content and amino acid composition. No such pool of Cu-metallothi...

Over six months 10 biochemically and physiologically atypical strains of Streptococcus pneumoniae were isolated from eye swabs. Conventional methodology showed that these strains possessed characteristics of both S pneumoniae and other alpha haemolytic streptococci. The use of sodium dodecyl sulphate - polyacrylamide gel electrophoresis (SDS-PAGE) enabled us to characterise these strains as S p...

Direct determination of the adsorbed amounts of surfactants on AgI by IR-spectroscopy is described. The adsorbed quantities are determined from the relationship between the intensities of the characteristic bands in IR-spectra of standard samples and the samples with adsorbed surfactants. The samples for IR-measurements are in the form of patterns consisting of AgI separated from the systems. T...

The capacities of 16 proteins, after being subjected to electrophoresis on polyacrylamide gel containing sodium dodecyl sulphate, to bind Coomassie Brilliant Blue G 250 have been measured. It is observed that absorption is a linear function of the surface area of the protein in spherical (globular) configuration. The dye binding capacity of proteins has been used to determine the molar ratio in...

The subunit of the Vicia graminea lectin with blood-group-N specificity was examined by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and gel filtration in 6M-guanidinium chloride, and its molecular weights was found to be 25 000. The unique N-terminal sequence fof the first nine residues of the lectin confirmed that Vicia lectin consists of four identical chains non-covalently lin...

The efficiency of direct conversion of tertiary alcohols bearing a β-hydrogen atom to vicinal halohydrins-chlorohydrins and bromohydrins-under green reaction conditions was tested preliminarily on model tertiary benzyl alcohols. Tertiary alcohols were successfully directly halogenated to vicinal halohydrins with N-halosuccinimide in aqueous media. The efficiency of the reaction in water was sig...

Alkaline treatment method was used to ext ract collagen. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and circular dichroism (CD) spectra revealed that the alkali-soluble pigskin collagen (ASPC) retained the polypeptide chains and triple helix conformat ion. The amino acid profiles showed no major deviation from the characteristic collagen composition. Both of the denat...

A simple method for identification and characterization of telomere-binding proteins is described in this article. After Sodium Dodecyl Sulphate-Polyacrylamide gel electrophoresis (SDS-PAGE), proteins are eluted, renatured and used for retardation analysis with labelled oligonucleotides corresponding to human and plant of telomeric sequences. We show here that this method is efficient to recove...