In this study, we developed rapid and sensitive assays for the detection of Cladophialophora carrionii, a common agent of human chromoblastomycosis. The isothermal techniques evaluated were rolling-circle amplification (RCA), multiplex ligation-dependent probe amplification (MLPA), and loop-mediated isothermal amplification (LAMP). The probes for RCA and MLPA were designed with target sequences...

The phylogenetic position of Saccharomycodes sinensis has been debated by yeast taxonomists. In this study, a multigene phylogenetic analysis based on four regions, namely the 18S ribosomal DNA (rDNA), the D1/D2 domains of the 26S rDNA, the second largest subunit of RNA polymerase II gene (RPB2) and translation elongation factor 1-α gene (EF1-α), were performed to address the phylogenetic place...

The Mediterranean islands are known as natural laboratories of evolution with a high level of endemic biodiversity. However, most biodiversity assessments have focused mainly on terrestrial and marine fauna, leaving the freshwater animals aside. Crete is one of the largest islands in the Mediterranean Basin, with a long history of isolation from the continental mainland. Gammarid amphipods are ...

The Elephant in the Room Until two decades ago, the notion that heart failure (HF) could occur in the presence of normal ejection fraction (EF) was met with great skepticism. While most physicians could recall encounters with hypertensive elderly ladies in acute pulmonary oedema despite a normal EF, it was not until large epidemiologic studies provided evidence for the existence of this syndrom...

BACKGROUND In environmental sequencing studies, fungi can be identified based on nucleic acid sequences, using either highly variable sequences as species barcodes or conserved sequences containing a high-quality phylogenetic signal. For the latter, identification relies on phylogenetic analyses and the adoption of the phylogenetic species concept. Such analysis requires that the reference sequ...

Slow and unreliable infection in the greenhouse has been a barrier to research on Armillaria root disease. The existing infection assay takes 7-18 months for detectable infection, during which time the inoculum often dies, resulting in unequal challenge among plants. Because symptom expression and mortality are rare, presence or absence of infection, determined by culturing, is the only datum d...

We investigate the query complexity of fair allocation indivisible goods. For two agents with arbitrary monotonic utilities, we design an algorithm that computes satisfying envy-freeness up to one good (EF1), a relaxation envy-freeness, using logarithmic number queries. show bound also holds for three additive and polylogarithmic utilities. These results suggest it is possible fairly allocate g...

Curvacin A, a type of bacteriocin produced by Lactobacillus curvatus LC05 has large spectrum of inhibition against human pathogenic, food spoilage microorganisms and many strains of Lactobacillus were isolated as a test organisms. The Curvacin A inhibited Enterococcus faecalis EF1 Escherichia coli NCTC 10418 but did not inhibit Candida albicans ATCC 10321. The antibacterial activity of the stra...