Three methods were compared for the detection of prunus necrotic ringspot virus in herbaceous and woody plants: DAS-ELISA, nonisotopic dot-blot hybridization and reverse transcriptional polymerase chain reaction (RT-PCR). When purified virus preparations were used, the detection limit of the RT-PCR technique was 1·28 pg mL whereas nonisotopic molecular hybridization and DAS-ELISA allowed detect...

BACKGROUND Hepatitis C virus infection is now one the common infection in Pakistan. Patients are routinely screened by antibody assays. Objective of this study was to assess the viremia in patients labelled as anti-HCV positive by ELISA. METHODS It this retrospective study patients labelled as anti HCV positive by ELISA were assessed for HCV RNA by polymerase chain reaction. The 254 HCV posit...

A sensitive duplex-immunocapture-RT-PCR (D-IC-RT-PCR) technique was developed for detection and discrimination of taxonomically distinct Sugarcane streak mosaic virus (SCSMV) and Sugarcane mosaic virus (SCMV) that naturally infect sugarcane. D-IC-RT-PCR was performed using polyclonal antisera for capture of virions. Oligo 5'-d(T)18(AGC)-3' as a common reverse primer for both viruses and virus s...

Rotavirus is the most common cause of severe diarrhea in newborn calves. In this study, it was aimed to investigate frequency of Rotavirus infections in calves in Kars region in Northeast Turkey by feces sampling 112 diarrheic calves of 0-6 months old. Feces samples were tested for Rotavirus antigen by Enzyme Linked Immunosorbent Assay (ELISA). Of the 112 feces samples examined by ELISA 10 samp...

The positive predictability of anti-HCV ELISA is low, especially, in blood donors and in healthy populations. False positive anti-HCV results pose some difficulties in medical practice and in blood screening. The aim of this study was to identify the factors associated with true hepatitis C virus (HCV) infection among anti-HCV ELISA-positives. A case-control analysis was conducted using 354 sub...

Bluetongue has emerged recently in Belgium. A bluetongue virus strain was isolated and characterized as serotype 8. Two new real-time reverse transcription-quantitative PCRs (RT-qPCRs) that amplified 2 different segments of bluetongue virus detected this exotic strain. These 2 RT-qPCRs detected infection earlier than a competitive ELISA for antibody detection.

Since April 2010, Tembusu virus (TMUV) which is a contagious pathogen of waterfowls, causing symptoms of high fever, loss of appetite and fall in egg production, has been reported in east of China. A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) which detects for TMUV was developed, using two monoclonal antibodies (mAbs) against the TMUV envelope (E) protein. BALB/c mic...

Outbreaks of the Southern rice black-streaked dwarf virus (SRBSDV) have caused significant losses in many rice-growing areas Vietnam, especially both North and Central Vietnam recent years. To detect virus, traditional reverse transcription polymerase chain reaction (RT-PCR) methodology immunoassays are currently employed. RT-PCR is accurate but requires expensive chemicals instruments, as well...

Aims: In this study, it was aimed to investigate the occurrence of cucurbit yellow stunting disorder virus (CYSDV), chlorotic yellows (CCYV) and beet pseudo-yellows (BPYV) in cucumber, zucchini melon plants grown Hatay province by Double Antibody Sandwich-Enzyme Linked Immuno Sorbent Assay (DAS-ELISA) Reverse Transcription-Polymerase Chain Reaction (RT-PCR) methods, analyse phylogenetic relatio...