BACKGROUND A systematic literature review of the last 40 years on the research of serum antibodies to Pseudomonas aeruginosa in cystic fibrosis and its utility as a diagnostic tool. METHODS Research papers in English, Portuguese, and Spanish were accessed through electronic databases (PubMed, Medline, LILACS, and SciELO). RESULTS 26 studies were assessed. ELISA technique was the most common...

AIM Determine the optimal cut-off and the use of Fluorescence Polarization Assay (FPA) to improve the detection of brucellosis in individual goats in Argentina. METHODS Sera from 96 goats from a flock with abortion due to B. melitensis biovar 1 were used to assess the efficacy of the FPA to detect brucellosis in goats. FPA results were compared with those of the Buffered Antigen Plate Aggluti...

Dot-ELISA, a technique that shares the same principles as the enzyme immunoassay, is useful for detection of anti-Schistosoma mansoni antibodies in the sera of patients with Schistosoma mansoni infections. The antigens were fixed to the nitrocellulose strips, blocked with 1% bovine serum albumin in 0.05% Tween 20. Patient sera (40) and normal laboratory personnel sera (9) were applied to the sh...

An enzyme-linked immunosorbent assay (ELISA) for the detection of Rickettsia typhi antigen in homogenates of pooled or individual laboratory infected fleas is described. The assay uses a double sandwich technique, employing a pool of monoclonal antibodies to capture the antigen and a hyperimmune rabbit serum for antigen detection. Using pools of R. typhi infected Xenopsylla cheopis, Ctenocephal...

to develop an elisa technique for the detection of antibodies against babesia ovis, the infectederythrocytes were lysed and the supernatant soluble antigen, after sonication and ultracentrifugation of thelysate was used as antigen. optimal dilution of the antigen was determined by checkerboard titrations, usingpositive and negative control sera. a correlation of 85% was observed between the res...

This study aimed to compare the sensitivity of multiplex PCR to ELISA technique in the instantaneous detection of HBV, HCV and HIVin blood samples from donors of the National blood Transfusion Centre in Togo. A total of 440 blood samplesfrom volunteer were collected and tested by ELISA and multiplex PCR for HBV, HCV and HIV detection. Among the 440 volunteer blood donors, 83% were female and 17...

MJ Martin, Met Office, Exeter, UK. M Balmaseda, ECMWF, Reading, UK L Bertino, NERSC, Bergen, Norway P Brasseur, LEGI, Grenoble, France G Brassington, Bureau of Meteorology, Melbourne, Australia J Cummings, NRL, Monterey, USA Y Fujii, JMA/MRI, Tsukuba, Japan DJ Lea, Met Office, Exeter, UK J-M Lellouche, Mercator Ocean, Toulouse, France K Mogensen, ECMWF, Reading, UK P Oke, CSIRO, Hobart, Austral...

In this paper, we employ the view model given by Bertino to propose a new design approach for a secure multi-level object-oriented database system. The central idea is to provide the user with a multi-level view derived from a single-level secure object-oriented database. Hence the database operations performed on the multi-level views are decomposed into a set of operations on the single-level...

At ISPEC ’12, Samelin et al. show that the redactable signature scheme introduced at VLDB ’08 by Kundu and Bertino does not always preserve the structural integrity of the tree signed. In particular, they show how redaction of non-leaves promotes descendants and allows a third party to add new edges to the signed tree. This alters the semantic meaning of the tree and is not acceptable in certai...

A crude antigenic preparation of Babesia bigemina was used to develop an ELISA for the detection of IgM antibodies. Optimal dilutions of the antigen, using positive and negative reference sera, were determined by checkerboard titrations. Negative sera from cattle imported from tick-free areas, serum samples collected from infected B. bigemina cattle were used to validate the test. The specifici...