ABSTRACT: The fluorescence polarization assay (FPA), two variants (V) of the indirect enzyme-linked immunosorbent (I-ELISA) and competitive (C-ELISA) were evaluated in buffaloes to detect antibodies against Brucella spp. V1 I-ELISA identifies them through monoclonal (M23) anti-bovine IgG (I-ELISAM23) V2 ProteinA / G (I-ELISA-A/G). Serum samples 862 (Bubalus bubalis) from Northeast Argentina (NE...

Background: Brucellosis is one of the endemic and a Zoonotic disease of the Iran. Science the rapid diagnosis and quick treatment are very important, selection of a sensitive and specific test solving this problem is of a great importance. The main aim of this study is comparison of routine serologic test with standard Elisa test. Materials and methods: In this survey, 176 suspected patient...

The intra-dermal tuberculin test is the standard test for the diagnosis of bovine tuberculosis [BTB] although it is not efficient in detecting all infected animals. This necessitates the need for a complementary testing scheme so as to maximize the detection of the BTB infection. The objective of this study was to evaluate Mycobacterium bovis [M. bovis] antibody test for the diagnosis of BTB. A...

The objective of this study was to compare the performance of different diagnostic tests for the detection of pigs subclinically infected with Actinobacillus pleuropneumoniae. To address this question, we infected pigs with APP serotypes 1, 3, 5, 7, 10, 12, and 15 and collected weekly tonsil swabs for isolation and PCR and serum samples for serological testing. Our results showed that the Multi...

An enzyme-linked immunosorbent assay (ELISA) for the detection of cattle antibodies to bovine herpesvirus type 1 was developed on the basis of competition between serum antibody and a virus-neutralizing mouse monoclonal antibody. The assay showed improved sensitivity over the virus neutralization (VN) test and over an enhanced VN test in which incubation of antibody-virus mixtures was carried o...

To study the transmission rate of hepatitis C virus (HCV) in the female sexual partners of antibody-positive hemophilic males, 106 partners from three hemophilia centers located in Europe, America, and Australia were tested for HCV seropositivity using a first-generation enzyme-linked immunosorbent assay (ELISA-1) and, subsequently, a second-generation ELISA (ELISA-2) and a supplemental recombi...