BACKGROUND The success of in vitro fertilization techniques is defined by multiple factors including embryo culture conditions, related to the composition of the culture medium. In view of the lack of solid scientific data and in view of the current general belief that sequential media are superior to single media, the aim of this randomized study was to compare the embryo quality in two types ...

Use of oocytes from prepubertal animals for in vitro embryo production holds potential application for reducing generation intervals and increasing genetic progress through embryo transfer. The objective of these studies was to compare the effect of three sperm pretreatments (prior to in vitro fertilization) and seven embryo culture protocols on fertilization rate and (or) subsequent developmen...

This review considers the relationship of in vitro gamete maturation and embryo culture to the future development of animal biotechnology. The areas reviewed are oocyte maturation in vitro and embryo culture and their importance for successful in vitro embryo production. The rapidly developing area of spermatogonial cell transplantation and culture is also reviewed. The scientific milestones le...

cumulative embryo score (ces) is one of the many embryo scoring methods which have been developed to help clinicians to transfer high quality embryos and predict pregnancy rate in assisted reproductive techniques (art) cycles. regarding the existing difference in ces calculation this study was done to compare two methods in order to determine the more practical and preferable one. in a retrospe...

Z.P. Nagy et al. (eds.), Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices, DOI 10.1007/978-1-4419-1780-5_19, © Springer Science+Business Media, LLC 2012 Abstract The INVO procedure using the INVOcell device is a simple technique of human assisted reproduction. The fertilization of the oocytes and the early development of the embryos occur in the INVOcell device pla...

Biotechnology can offer useful tools to complement conventional breeding programs. The techniques of in vitro selection, that exploit phenomenon, called somaclonal variation, by screening cell cultures for resistance to herbicides, different types of stress or diseases, already helped to obtain tolerant lines of flax (Linum usitatissimum), which were used in further commercial breeding programs...

Uridine diphosphate (UDP) glucuronyltransferase activity in chick liver rises at hatching from near zero to adult levels. This rise will occur prematurely in embryo liver during organ culture. Increase in enzyme activity during organ culture differs with embryo age: in liver from 11-day old embryos it ceases at adult values; in liver from 5-day old embryos it continues to much higher-than-adult...

Over the years it has been an important goal to optimize the treatment of subfertility in order to obtain a better pregnancy rate and baby-take-home rate, usually combined with the goal of decreasing the rate of multiplet pregnancies. This implies optimizing the development of embryos through improved in vitro culture methods. And it also implies optimizing the methods of selection of embryos f...

plant tissue culture is an alternative method of commercial propagation and is being used widely for the commercial propagation of a large number of plant species, including many medicinal plants. somatic embryogenesis is a process by which asexual or somatic cells are induced to form embryos in culture. somatic embryogenesis is a multi-step regeneration process starting with formation of pro-e...

Cell Suspension Culture, Somatic Embryo Genetic and Synthetic Seed Production in Different Cultivars of Potato