The purpose of this study is to develop a mouse sperm preservation method based on evaporative drying. Mouse sperm were evaporatively dried and stored at 4°C and ambient temperature for 3 months to 2 years. Upon rehydration, a single sperm was injected into a mature oocyte to develop into a blastocyst after culture or a live birth after embryo transfer to a recipient female. For the samples sto...

Reproductive technology is advancing at a steadfast pace. Researchers are successfully refining options for fertility preservation, to the benefit of the cancer community. Research has consistently shown cancer patients and survivors desire to have risks to fertility and preservation options disclosed, and major campaigns have been undertaken to refer these patients to fertility specialists. Ho...

Introduction Causes of impaired female reproductive function Environmental Endocrine Disrupters and Ovarian Function Indications of reproductive function preservation Choice of the method for ovarian function preservation Cryopreservation of Oocytes Introduction The need for oocyte cryopreservation Cryobiological factors influencing cryoefficiency Oocyte structures affected by cryopreservation ...

In vitro plant tissue culture and biotechnology used to assist support the development of breeding when classical methods propagation must be accelerated or it was necessary overcome barriers inaccessible by approaches. asparagus, improve multiple tasks, a high number in have been used, such as regeneration through organogenesis, embryogenesis, manipulation ploidy, protoplast isolation, genetic...

Purpose. To investigate the prevalence of and experience related to infertility fertility preservation during training for United States (US) medical residents fellows. Methods. Cross-sectional online-based survey study US postgraduate Results. Respondents included 732 fellows, with highest percentage in Obstetrics & Gynecology (24.2%), Pediatrics (14.1%), Internal Medicine (13.9%). In total, o...

Continuous, long-term observations of amniote embryos have always been difficult. Special culture techniques for young avian and mammalian embryos have been developed (New, 1967) and these have helped to visualize the early stages of development. But studies of normal and abnormal development during the major period of organogenesis have been made largely by tedious indirect methods, such as th...

BACKGROUND Current options for female fertility preservation in the face of cytotoxic treatments include embryo, oocyte and ovarian tissue cryopreservation. However these methods are limited by the patient age, status or available timeframe before treatment and they necessitate invasive procedures. Agents which can prevent or attenuate the ovotoxic effects of treatment would provide significant...

BACKGROUND A successful oocyte cryopreservation programme is of utmost importance where a limited number of oocytes can be inseminated per cycle, to overcome legal and ethical issues related to embryo storage, for oocyte donation programmes and for fertility preservation (especially for cancer patients). Vitrification has been recently proposed as an effective procedure for this purpose. METH...

PURPOSE To update guidance for health care providers about fertility preservation for adults and children with cancer. METHODS A systematic review of the literature published from March 2006 through January 2013 was completed using MEDLINE and the Cochrane Collaboration Library. An Update Panel reviewed the evidence and updated the recommendation language. RESULTS There were 222 new publica...

In this article we review the existing fertility preservation options for women diagnosed with Turner syndrome and provide practical guidelines for the practitioner. Turner syndrome is the most common sex chromosome abnormality in women, occurring in approximately 1 in 2500 live births. Women with Turner syndrome are at extremely high risk for primary ovarian insufficiency and infertility. Alth...