To explore the genetic diversity, host specificity, and zoonotic potential of Enterocytozoon bieneusi, feces from 348 stray and pet dogs and 96 pet cats from different locations in China were examined by internal transcribed spacer (ITS)-based PCR. E. bieneusi was detected in 15.5% of the dogs, including 20.5% of stray dogs and 11.7% of pet dogs, and in 11.5% of the pet cats. Higher infection r...

24 To explore the genetic diversity, host specificity and zoonotic potential of Enterocytozoon 25 bieneusi, feces from 348 stray and pet dogs, and 96 pet cats from different locations in China 26 were examined by internal transcribed spacer (ITS) based PCR. E. bieneusi was detected in 27 15.5% of dogs including 20.5% of stray dogs and 11.7% of pet dogs, and in 11.5% of pet 28 cats. Higher infec...

Abstract The current study presents a comprehensive review of worldwide waterborne parasitic protozoan outbreaks reported between 2017 and 2022. Totally, 416 were attributed to transmission protozoa. Cryptosporidium was the etiological agent in 77.4% (322) outbreaks, while Giardia 17.1% (71). Toxoplasma gondii Naegleria fowleri causal 1.4% (6), 1% (4) respectively. Each Blastocystis hominis, Cy...

Several hybridomas producing antibodies detected by indirect immunofluorescence antibody test (IFAT) were established by fusion of mouse myeloma SP2/O with spleen cells from BALB/c mice immunized against whole spores (protocol 1) or chitinase-treated spores (protocol 2) of Enterocytozoon bieneusi and were cloned twice by limiting dilutions. Two monoclonal antibodies (MAbs), 3B82H2 from protocol...

BACKGROUND Enterocytozoon hepatopenaei (EHP) causes hepatopancreatic microsporidiosis (HPM) in shrimp. It is probably endemic in Australasia and was first characterized and named from the giant or black tiger shrimp Penaeus monodon from Thailand in 2009. Later, it was also found to infect exotic Penaeus vannamei imported for cultivation in Asia. HPM is not normally associated with shrimp mortal...

A total of 219 and 124 individual fecal samples of horses and donkeys, respectively, were screened for the presence of Cryptosporidium spp., Encephalitozoon spp., and Enterocytozoon bieneusi DNA by genus-specific nested PCR. Isolates were genotyped by sequence analysis of SSU rRNA, GP60, TRAP-C1, COWP, and HSP70 loci in Cryptosporidium, and the ITS region in microsporidia. Cryptosporidium spp. ...