Detection and Frequency of Enterotoxin (cpa,cpe) Genes Clostridium perfringens Isolated from Dehydrated Vegetables by PCR

Clostridium perfringens type C isolates cause enteritis necroticans in humans or necrotizing enteritis and enterotoxemia in domestic animals. Type C isolates always produce alpha toxin and beta toxin but often produce additional toxins, e.g., beta2 toxin or enterotoxin. Since plasmid carriage of toxin-encoding genes has not been systematically investigated for type C isolates, the current study...

A novel multiplex PCR method using three sets of specific primers was developed for the detection of the cytotoxic (act), heat-labile (alt), and heat-stable (ast) enterotoxin genes in Aeromonas spp. This assay was used to characterize 35 reference strains as well as 537 food-borne isolates. A total of seven gene pattern combinations were encountered, including act, alt, act/alt, act/alt/ast, ac...

Abstract Background Staphylococcus aureus is an important opportunistic pathogen of raw milk and products, the enterotoxins cause food poisoning. Milk products are reservoirs enterotoxin-producing S. . The aims this systematic review were to estimate pooled prevalence , including methicillin-resistant (MRSA), summarize their molecular characteristics, assess potential sources contamination in b...

yersinia enterocolitica is newly included in the classification of both the invasive bacteria, e.g. shigella, and the toxicogenic bacteria, e.g. escherichia coli. five human strains and 45 environmental strains were used to produce y.enterocolitical enterotoxin at 40c, 240c and 370c using infant mouse assay. at 250c enterotoxin was produced by 10 environmental strains and by all of the 3 human ...

Background: An annual update of information about the prevalence of Staphylococcus aureus and staphylococcal enterotoxins (SEs) genes is required in every geographic area. Aims: This study was conducted to investigate the prevalence of the bacterium and type of associated enterotoxin genes in different food samples, using multiplex polymerase ...

VYLETĚLOVÁ, M., BANYKÓ, J.: Detection of coding genes for enterotoxins in Bacillus cereus by PCR and their products by BCET-RPLA and ELISA Assay. Acta univ. agric. et silvic. Mendel. Brun., 2010, LVIII, No. 5, pp. 417–426 Determination of enterotoxin production, diarrhoeal and emetic gene identifi cation was studied in 41 Bacillus cereus strains isolated from raw cows’ and raw goats’ milk, past...

Polymerase chain reaction assays and culture were used to investigate 728 faecal samples from 404 calves (286 diarrhoeic, 118 healthy) and 324 lambs (230 diarrhoeic, 94 healthy) in Kashmir, India, for the presence of enterotoxigenic Escherichia coli (ETEC), enteroaggregative E. coli (EAEC), diffusely adherent E. coli (DAEC) and salmonellae. Antimicrobial sensitivity patterns were also investiga...