Recently, mesoporous carbon nitride (MCN) has aroused extensive interest for its potential applications in organocatalysis, photo- and electrochemistry and CO2 capture. However, further surface functionalization of MCN for advanced nanomaterials and catalysis still remains very challenging. Here we show that acidic carboxyl groups can be smoothly introduced onto the surface of well-ordered MCN ...

Conventional fermentation technologies are limited due to low reactor productivity, low product yield, and low product concentration mainly due to product inhibition. There are several ways of improving the overall behavior of a biotechnological process. The cells can be modified so that the product is excreted prior to reaching inhibiting concentrations. Furthermore, a selective mutation of th...

The design of biocatalytic processes for industrial synthetic chemistry is determined in large part by the choice of isolated enzyme or whole-cell catalyst form. In the present paper, the considerations for choice are identified and some important classes of bioconversion are discussed in relation to the choice to be made. Recent developments in cell and protein engineering as well as reactor a...

Agarose-vinyl sulfone (VS) beads have proven to be a good support immobilize several enzymes. However, some enzymes are hardly immobilized on it. This is the case of penicillin G acylase (PGA) from Escherichia coli, which very slowly this (less than 10% in 24 h). enzyme also not significantly adsorbed aminated MANAE-agarose beads, an anionic exchanger. In study, were modified with divinyl (DVS)...

While Nature excels at performing selective modifications of complex polyfunctional molecules through the use of tailoring enzymes, synthetic chemistry has lagged behind in this regard. In prior work, we have applied a biomimetic approach to this problem, developing small peptides to achieve various group transfer reactions on polyol substrates with high enantio- or regioselectivity. The utilit...

A self-assembled supramolecular complex is reported to catalyze alkyl-alkyl reductive elimination from high-valent transition metal complexes [such as gold(III) and platinum(IV)], the central bond-forming elementary step in many catalytic processes. The catalytic microenvironment of the supramolecular assembly acts as a functional enzyme mimic, applying the concepts of enzymatic catalysis to a ...

Molecular nanoprobes with intrinsic enzyme-like activity represent a new wave of technology for rapid and sensitive detection molecular targets. This work reports synthesis characterization novel well-dispersed europium-doped ceria nanocrystals (EuCe NCs) self-integrated catalytic fluorescence sensing functions. The NCs have an average size ∼5 nm exhibit bright stable more than 6 months in aque...

a simple and efficient one-pot three-component synthesis of the biologically important spirooxindole-4h-pyrans was carried out by the reaction of isatin, activated methylene reagents, and 4-hydroxycoumarin in aqueous medium. sba-pr-nh2 was found to be an efficient heterogeneous nanoporous solid basic catalyst (pore size of 6 nm) which can be easily handled and removed from the reaction mixture ...

Deuterium-labeled nicotinamide cofactors such as [4-2H]-NADH can be used mechanistic probes in biological redox processes and offer a route to the synthesis of selectively [2H] labeled chemicals via biocatalytic reductive deuteration. Atom-efficient routes formation recycling are therefore highly desirable but require careful design order alleviate requirement for [2H]-labeled reducing agents. ...

The scientific community recognizes coffee grounds (Coffea arabica) as an important biological residue, which led to using the Eversa® Transform 2.0 lipase in silico enzymatic catalyst for grounds’ free fatty acids (FFA). Molecular modeling studies, including molecular docking, were performed, revealed structures of and showed primary interactions between ligands amino acid residues active site...