Abstract Diabetes mellitus is a metabolic disease characterized by hyperglycemia resulting from either defect in insulin secretion, insulin action, or both. Postprandial hyperglycemia is a prime characteristic of diabetes mellitus and has been a focus in the therapy for diabetes. One of the therapeutic approaches which involve decreasing hyperglycemia aims at lowering blood glucose...

Porous copolymer was prepared by suspension polymerization method of styrene, maleic anhydride and divinylbenzene in a batch type reactor. The characteristics of the copolymer were evaluated using Fourier Transform IR spectroscopy and scanning electron microscopy. The glucose oxidase (GOD) enzyme was selected as a model enzyme to assess its potential applicability for immobilization purpose. Th...

This study investigated the effectiveness of immobilizing Saccharomyces cerevisiae invertase (SInv) on magnetite nanoparticles to produce fructooligosaccharides (FOSs). Based existing literature and accompanied by parameter estimation, a modified kinetic model was employed represent kinetics sucrose hydrolysis transfructosylation using SInv immobilized nanoparticle surfaces. utilized simulate p...

Here, a low-cost glucose/O 2 Y-shaped microfluidic biofuel cell was developed using printed circuit board for microelectrode construction. A double-side tape based on the pressure-sensitive adhesive used microchannel fabrication. nanocomposite that consisted of reduced graphene oxide gold nanoparticles (AuNPs), and poly neutral red connected to enzymes applied copper electrode surface. The Aspe...

Highly cross-linked polyethylene glycol monoliths (HCPEG) with interconnected micro and nanoporosity are produced via photo-crosslinking of low internal phase emulsions (LIPE). Unlike previous works, this approach allows the pre-processing functionalization both polymer matrix porosity by loading phases emulsion template several active fillers, such as enzymes, semiconductive polymers, metallic...

Glucose oxidase and catalase were immobilized on poly(diethylaminoethyl methacrylate-g-ethylene glycol) gels by copolymerization of the constituent monomers and the functionalized enzyme solutions. The hydrogels were prepared in the form of discs and microparticles. The amount and the activity of enzymes immobilized in the matrix were determined. The hydrogels were tested for their response to ...

DeazaFMN-containing glycolate oxidase has been prepared and shown to catalyze the stereospecific transfer of the alpha-hydrogen from substrate to enzyme-bound deazaFMN. The reaction of sulfite, cyanide, and hydroxylamine with several deazaflavin-containing enzymes (glycolate oxidase, D-amino acid oxidase, glucose oxidase, N-methylglutamate synthetase) and free deazaFMN has been examined. All th...

introduction: oxalate oxidase (ec.1, 2, 3, 4), an acute phase protein of plants, is used widely in agriculture, food industries, medical diagnosis of kidney stones and oxaluria. in this study oxalate oxidase from barley was purified and partially characterized. materials & methods: roots and sprouts of barley cultured in hydroponic states were separated and extracted. ion exchange resins of the...