Methods Our goal was to study the relationship between pregnant women diagnosed with acute toxoplasmosis and the presence of this parasite in their house pets. Using chemiluminescence enzyme immunoassay (CLIA) we determined the presence of toxoplasma IgM and IgG antibodies and by enzyme immunoassay (EIA) toxoplasma IgA antibodies. Enzyme-linked immunosorbent assay (ELISA) was used to determine ...

The diagnostic gains of repeat testing for Clostridium difficile by enzyme immunoassay and PCR (i.e., initial negative result followed by positive result) within a 7-day period were 1.9 and 1.7%, respectively. There is little value of repeat testing for C. difficile by enzyme immunoassay or PCR.

The Directigen FLU-A enzyme immunoassay for the detection of influenza A virus was compared with direct smear and culture in 211 clinical specimens. The FLU-A enzyme immunoassay proved to be a reliable, rapid screen for influenza A from symptomatic patients and was less dependent on technical expertise for interpretation than were direct smears.

A surveillance system used different detection methods to estimate prevalence of Shiga toxin-producing Escherichia coli during 2003-2005 and 2001-2002. More non-O157 serotypes were detected by enzyme immunoassay than by evaluation of non-sorbitol-fermenting E. coli isolates. We therefore recommend use of enzyme immunoassay and culture-based methods.

Sera from patients being treated with phenytoin were analyzed for the drug by spectrophotometry, gas chromatography, radioimmunoassay, enzyme immunoassay, and liquid chromatography. The essay values obtained were intercompared statistically. Enzyme immunoassay and liquid chromatography appear to be attractive alternatives to the more traditional methods of spectrophotometry and gas chromatograp...

We assessed Calcofluor white staining, Aspergillus polymerase chain reaction, and a galactomannan enzyme immunoassay for diagnosis of fungal infection with use of computed tomography-guided percutaneous lung biopsy specimens obtained from 61 patients. The sensitivity and specificity of computerized tomography, Aspergillus polymerase chain reaction, and galactomannan enzyme immunoassay were 100%...

A commercially available luminescence enhanced enzyme immunoassay (Amerlite--Amersham International) for carcinoembryonic antigen (CEA) was compared with an established enzyme immunoassay (Monoclonal 1-step Assay--Abbott Laboratories). A reference range for healthy blood donors (n = 272) was established for both kits. The blood donors were not separated into smokers and non-smokers, but were ex...