The exopolysaccharide secreted by Lacticaseibacillus paracasei subsp. SS-01 strain (LP-EPS) is isolated and purified from yogurt. It a polysaccharide with branched multi-stranded structure, which exists in smooth rod-like or cloud-like state, possesses good thermal stability molecular weight of 49.68 kDa (±4.436%). LP-EPS shows high antioxidant capacity, anti-inflammatory anti-sensitizing activ...

One of the primary goals antiviral research in recent decades has been biosynthesis new agents. The large number bioactive agents extracted from microbial isolates led to building connections between products through screening. Using a genotype 4 plasmid, extract fraction Pseudomonas oleovorans evaluated against HCV Hepatitis C cell culture. cytotoxicity assay using MTT [3-(4, 5-dimethylthiazol...

A highly sensitive and specific enzyme inhibition assay based on alcohol oxidase (AlOx) and horseradish peroxidase (HRP) for determination of mercury Hg(II) in water samples has been presented. This article describes the optimization and miniaturization of an enzymatic assay using a chemiluminescence reaction. The analytical performance and detection limit for determination of Hg(II) was optimi...

Previous investigation discovered a phenylpropanoid compound called (2E,4E)-1,5-diphenylpenta-2,4-dien-1-one in the chloroform fraction of Sterculia quadrifida seeds. This  exhibited notable cytotoxic effects, displaying IC50 values 2.29, 9.93, 18.09, and 12.12 µg/mL 4T1, MCF-7, MDA-MB-435, T47D breast cancer cell lines respectively. In our current study, we performed an MMP-9 enzyme inhib...

Skin diseases can get natural therapies from medicinal plant-based products. In this study, a topical cream was formulated ethanol extract of Acacia sieberiana, beeswax and Vitellaria paradoxa (shea) butter. GC-MS characterization with co-injection the revealed Stearic acid (31.43%), Palmitic (23.15%), Oleic (21.44%) Linoleic (16.20%) as major components. Seven phenolic conpounds were identifie...

The plant Cardaria draba (L.) Desv. subsp. (Brassicaceae), is traditionally used for various ailments in different cultures. We investigated solvent extracts such as methanol, ethanol, and water obtained from flowers, leaves, stems, roots of C. their chemical composition, well antioxidant enzyme inhibition activities acetylcholinesterase (AChE), butyrylcholinesterase (BChE), ?-glucosidase, ?-am...

The nasal decongestants oxymetazoline and xylometazoline are frequently used in the topical treatment of rhinitis and sinusitis. As nitric oxide (NO) is thought to play a role in inflammation of the upper respiratory tract, the aim of this study was to examine the in vitro effects of these compounds on the activity and the expression of NO producing enzymes, including the inducible form of NO s...

A higher throughput method of screening for the inhibition of recombinant CYP2D6 using a microtiter plate (MTP) assay was evaluated using 62 new chemical entities and compared to data from the dextromethorphan O-demethylase assay in human liver microsomes (HLM). The IC50 values for the two assays closely matched for 53 compounds (85%). Six of the variant nine compounds had higher IC50 values wi...

An assay technique, homogeneous enzyme immunoassay, is described for the quantitative determination of morphine derivatives in biological fluids. An enzyme is labeled with morphine. When the enzymelabeled morphine is bound by antimorphine antibodies, the enzyme is rendered inactive. Free morphine competes with enzyme-morphine for antibody binding sites preventing inhibition of enzyme activity. ...

The specificity of the enzyme-linked immunosorbent assay(s) is thought to depend on the specificity of the antibody used in the assay system. Therefore, the association of broadly reactive antigens like endotoxin with enzyme conjugates or other enzyme-linked immunosorbent assay reagents has the potential of altering the specificity of reactions in the enzyme-linked immunosorbent assay. Using th...