the mechanism by which bi-and trivalent cations affect human liver phosphatidatephosphohydrolase (pap) activity was investigated. bivalent cations up to 1 mm increased pap activity whereas at higher concentrations the activity of the enzyme decreased. the stimulatory concentration for trivalent cations such as al3+ and cr3+, however, was much lower being 2 m m and 1 m m, respectively. all catio...

Artificial cell-like communities participate in diverse modes of chemical interaction but exhibit minimal interfacing with their local environment. Here we develop an interactive microsystem based on the immobilization a population enzyme-active semipermeable proteinosomes within helical hydrogel filament to implement signal-induced movement. We attach large single-polynucleotide/peptide microc...

Acriflavine (3,6-diaminoacridine) is an anticeptic drug developed in 1912. Previous research has focused on investigation of the intercalating features of acriflavine, but little is known about its interaction with proteins. Drug-receptor interaction is of major interest in clinical science. The aim of the present study was to evaluate the ability of acriflavine to induce alterations in conform...

The structure and function of an enzyme can be altered by nanoparticles (NPs). The interaction between enzyme and NPs is governed by the key properties of NPs, such as structure, size, surface chemistry, charge and surface shape. Recent representative studies on the NP-enzyme interactions and the regulation of enzyme activity by NPs with different size, composition and surface modification are ...

The electron acceptor 2,6-dichlorophenolindophenol (DCI) forms a spectrophotometrically and kinetically detectable complex with bovine milk xanthine oxidase. The visible absorption spectrum of complexed DC1 exhibits an absorption maximum at 660 nm at pH 7.8 (E = 34.2 X lo3 M-’ cm-‘), a marked bathochromic shift from the visible absorption maximum at 608 nm characteristic of the free dye (E = 21...

the thermal denaturation of adenosine deaminase (ada) has been investigated in the presence of sodium n-dodecyl sulphate (sds) over the temperature range of (293-363k) in 2.5 mm phosphate buffer, ph 6.4 by temperature scanning spectroscopy. the interaction of sds caused the folding of adenosine deaminanse resulting in a decrease of th (temperature of minimum solubility), ts (temperature of maxi...

Abstract Targeting the interaction between severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2)-receptor-binding domain (RBD) and angiotensin-converting enzyme (ACE2) is believed to be an effective strategy for drug design inhibit infection of SARS-CoV-2. Herein, several ultrashort peptidase inhibitors against RBD–ACE2 were obtained by a computer-aided approach based on RBD-binding resi...