Four different ceria supported catalyst were prepared by impregnation method with Pt(NO3)2 solution. The two supports are commercially available (MaTeck) and the other two were prepared by precipitation and microwave assisted hydrothermal method (MAH) respectively. The phase composition and average crystallite size of the catalysts were characterised with XRD technique. Finally the catalytic ac...

a-amylase was immobilized on alumina via adsorption. The support and the immobilized enzymes were characterized using XRD, IR spectra and N2 adsorption studies. The efficiency of immobilized enzymes for starch hydrolysis was tested in a batch reactor. The effect of two different calcination temperatures on properties of the support as well as on immobilization was studied. From XRD, IR and N2 a...

We describe the use of immobilized enzymes in assay methods for the determination of glucose with glucose oxidase, uric acid with uricase, and urea with urease in serum samples. The enzyme reactor tubes were adapted to continuous-flow analyzers (Technicon AA II, SMA 12/60, and SMAC) used in routine laboratory determinations, and results with their use were compared to those from assays involvin...

The use of enzymes to catalyze chemical reactions has increased these recent years. Several models have been developed express the kinetics over biocatalysts. most well-known them, Michaelis-Menten, is used when only one substrate adsorbs on enzyme. In case esterification reaction, i.e., bimolecular system, a more complex kinetic model such as Ping-Pong Bi-Bi should be applied. advanced essenti...

In this paper, a process for the stable production of low allergenicity hydrolysates is presented. Whey protein was hydrolysed at 50 C and pH 8.5 using a bacterial protease in a continuous stirred tank membrane reactor including a polyethersulfone plate and frame ultrafiltration module with a molecular weight cut-off of 3 kDa. The reactor was maintained in operation for 16 h. Conversion reached...

To develop an intelligent sensor-effector functionality on the nanoscale, a pH-switchable, controlled nanoreactor based on amphiphilic copolymer membranes was built. The nanovesicles were equipped with bacterial transmembrane ompF pore proteins and the pH-sensitive enzyme acid phosphatase, resulting in a switchable substrate processing at pH 4-6.5. Ideal pH and substrate concentrations for the ...

Ionic liquid (IL) surfactant choline dioctylsulfosuccinate, [Cho][AOT], formed polydispersed vesicular structures in the IL, ethylmethylimidazolium ethylsulfate, [C2mim][C2OSO3]. Cytochrome c dissolved in such a colloidal medium has shown very high peroxidase activity (∼2 times to that in neat IL and ∼4 times to that in an aqueous buffer). Significantly, the enzyme retained both structural stab...

The design of biocatalytic processes for industrial synthetic chemistry is determined in large part by the choice of isolated enzyme or whole-cell catalyst form. In the present paper, the considerations for choice are identified and some important classes of bioconversion are discussed in relation to the choice to be made. Recent developments in cell and protein engineering as well as reactor a...

In this paper, we develop plug flow reactor models that simultaneously investigate how reaction and absorption, morphological differences, and temperature influence nutrient acquisition rates in simple, tubular animal guts. We present analytical solutions to the resulting reaction-advection equations that model these processes, and we obtain formulas giving the throughflow speed that maximizes ...

Conventional fermentation technologies are limited due to low reactor productivity, low product yield, and low product concentration mainly due to product inhibition. There are several ways of improving the overall behavior of a biotechnological process. The cells can be modified so that the product is excreted prior to reaching inhibiting concentrations. Furthermore, a selective mutation of th...