The objective of the study was to develop an immunohistochemical (IHC) assay for rapid detection of Erysipelothrix rhusiopathiae. Serotypes 1a, 1b, and 2 are most frequently associated with clinical disease in pigs. Antiserum against serotypes 1a, 1b, and 2 was produced in rabbits, pooled, and applied to formalin-fixed, paraffin-embedded tissue sections of pigs (lungs, heart, spleen, and skin)....

Erysipelothrix rhusiopathiae was isolated from tonsils of 63 (10.5%) of 600 apparently healthy slaughter pigs in the Kanto area of Japan in February and July 1984. The isolation rate was significantly higher during July than in February. Of these 63 isolates, 34 isolates (54.0%) were serotype 7, 20 isolates (31.7%) were serotype 2, 6 isolates (9.5%) were serotype 6, and 1 isolate (1.6%) each wa...

BACKGROUND Erysipelothrix rhusiopathiae is an established animal pathogen, which may cause infections in humans. It is a gram-positive rod and found in the tonsils or the digestive tracts of animals. The bacterium is occupationally related, as usually only people with frequent animal contacts are infected. We report a case of a patient who was admitted with an infectious tenosynovitis with bloo...

To clarify the role of surface protective antigen A (SpaA) in the pathogenesis of Erysipelothrix rhusiopathiae C43065 (serotype 2), the spaA deletion mutant of E. rhusiopathiae ΔspaA was constructed by homologous recombination. The virulence of the ΔspaA mutant decreased more than 76-fold compared with that of the wild-type strain C43065 in mice. The mutant strain was sensitive to the bacterici...

The production of antisera against E. rhusiopathiae, the causative agent of swine erysipelas and human erysipeloid, has been hindered by the poor growth of the organism in media not enriched with blood serum. The data on its growth requirements, reviewed by Karlson and Merchant (1941), are limited to descriptions of behavior in routine media. An analysis of its growth essentials was therefore i...