Four positively-charged residues, namely betaLys-155, betaArg-182, betaArg-246, and alphaArg-376 have been identified as Pi binding residues in Escherichia coli ATP synthase. They form a triangular Pi binding site in catalytic site betaE where substrate Pi initially binds for ATP synthesis in oxidative phosphorylation. Positive electrostatic charge in the vicinity of betaArg-246 is shown to be ...

The structure and dynamics of rat apo-cellular retinol binding protein II (apo-CRBP II) in solution has been determined by multidimensional NMR analysis of uniformly enriched recombinant rat 13C, 15N-apo-CRBP II and 15N-apo-CRBP II. The final ensemble of 24 NMR structures has been calculated from 3274 conformational restraints or 24.4 restraints/residue. The average root-mean-square deviation o...

BACKGROUND Sonoran ethnic groups (Yaquis, Mayos, Seris, Guarijíos, Pimas, Kikapúes and Pápagos) use mainly herbal based preparations as their first line of medicinal treatment. Among the plants used are those with anti-tuberculosis properties; however, no formal research is available. METHODS Organic extracts were obtained from nine medicinal plants traditionally used by Sonoran ethnic groups...

BACKGROUND The efficacy of essential oil of Chenopodium ambrosioides flowering aerial parts and its three main active ingredients was evaluated against Blattella germanica male adults. METHODS Composition of essential oil was determined by GC-MS. Topical application bioassay was used to evaluate contact toxicity of essential oil and three main components. Fumigant toxicity of essential oil an...

Introduction. Erysiphe cichoracearum /Ec/ is the predominating powdery mildew species on cucurbits in the Czech Republic (5). Its occurrence and spreading on cucurbits can be influenced not only by virulence, but also by aggressiveness of isolates within natural pathogen populations. Both forms of pathogenicity have an impact on plant cultivation and protection and should be considered in resis...

Powdery mildew on flowers of Cassia fistula and folioles Tamarindus indica, caused by Erysiphe quercicola, is reported for the first time in Brazil. The identity pathogen was confirmed morphological characteristics molecular sequence analyses ITS 28S rDNA regions. This report E. quercicola causing powdery C. T. indica