Determination of the stoichiometry of macromolecular assemblies is fundamental to an understanding of how they function. Many different biophysical methodologies may be used to determine stoichiometry. In the past, both sedimentation equilibrium and sedimentation velocity analytical ultracentrifugation have been employed to determine component stoichiometries. Recently, a method of globally ana...

Serum C-reactive protein (CRP) and the erythrocyte sedimentation rate (ESR) were compared in 241 patients with rheumatoid arthritis (RA). There was a positive linear correlation between the 2 measurements with a high degree of variability. Neither age nor duration of RA had a detectable influence. The relationship between CRP and ESR was, however, altered by treatment with gold, penicillamine, ...

The purification of human neutrophils for in vitro studies is challenging as they are easily activated through ex vivo manipulations. The technique of erythrocyte sedimentation combined with density gradient centrifugation remains widely practiced and was the subject of this study. Since in the sedimentation step the leukocytes are incubated with dextran, we have raised the likelihood that cell...

AIM Almost 20 different prognostic factors have so far been investigated in childhood bone tumors, but apart from clinical stage the results have been inconclusive. We evaluated possible prognostic factors of malignant bone tumors in children and adolescents. METHODS Seventy children and adolescents, who have been treated for bone sarcomas (36 with osteosarcoma and 34 with Ewing's sarcoma) at...

OBJECTIVES Our objective was to explore inflammation, measured as erythrocyte sedimentation rate (ESR), as a predictor for the development of heart failure (HF). BACKGROUND In recent years, evidence of the importance of inflammation in the pathophysiology of HF has emerged, and various inflammatory markers have been found to predict future HF. Erythrocyte sedimentation rate is an inexpensive ...

Tuberculosis of the clavicle is a rare lesion, accounting for less than 1% of all osteoarticular tuberculosis. The lesion has been described in a few case reports. The lesion can have a varied presentation, ranging from dull, aching pain to bony swelling and a draining sinus. The rarity of the lesion, its nonspecific symptoms, and its striking resemblance to common cystic conditions such as bon...

Thanks to the precise core dating and the high sedimentation rate of the drilling site (Gallipoli Terrace, Ionian Sea) we were able to measure a foraminiferal δ(18)O series covering the last 2,200 years with a time resolution shorter than 4 years. In order to support the quality of this data-set we link the δ(18)O values measured in the foraminifera shells to temperature and salinity measuremen...

Sedimentation velocity analysis is a powerful tool for the investigation of biological macromolecules under a wide range of solution conditions. If carefully applied, it can be an effective tool for the characterization of interacting systems in solution. It is rapidly becoming a method of choice among the biotechnology community. In recent years, there have been notable advances in the ease of...

The influence of sedimentation rates on biogeochemistry of riparian forests was studied near ephemeral streams at Fort Benning, GA. Upper reaches of seven ephemeral streams had received varying rates of sedimentation stemming from erosion along unpaved roadways at the military installation. Two reference catchments were also included in the study. Decomposition of foliar litter, microbial C and...

Mutants of Escherichia coti that fail to show the characteristic increase in permeability induced by brief treatment with ethylenediaminetetraacetate have been isolated. They were examined to see whether they release lipopolysaccharide as expected during such a treatment. Mutants derived from two strains released only 20 to 40% less lipopolysaccharide than their parents, but further analysis of...