Background: Cutaneous leishmaniasis (CL) is a serious public health problem in many tropical countries. The infection is caused by a protozoan parasite of Leishmania genus transmitted by Phlebotominae sandflies. In the present study, we constructed a eukaryotic expression vector to produce a fusion protein containing LmSTI1 from Leishmania major (L. major) and PpSP42 from Phlebotomus papatasi (...

background: hepatitis delta virus (hdv) is a subviral human pathogen that exploits host rna editing activity to produce two essential forms of the sole viral protein, hepatitis delta antigen (hdag). editing at the amber/w site of hdv antigenomic rna leads to the production of the large form (l-hdag), which is required for rna packaging. methods: in this study, pcr-based site-directed mutagenesi...

Abstract Background Hepatocellular carcinoma (HCC) is one of the most common cancers with high incidence and mortality. However, underlying mechanisms HCC still remain unclear. Eukaryotic translation initiation factors (eIFs) have a substantial effect on tumor development. In this study, we were aimed to investigate role eukaryotic factor 4 gamma 2 (EIF4G2) in HCC. Methods Western blot (WB) 30 ...

Gene expression in eukaryotes begins with transcription the nucleus, followed by synthesis of messenger RNA (mRNA), which is then exported to cytoplasm for its translation into proteins. Along and translation, mRNA export through nuclear pore complex (NPC) an essential regulatory step eukaryotic gene expression. Multiple factors regulate hence Interestingly, proteins from certain types viruses ...

Molecular farming technology offers a unique advantage that almost any protein can be produced economically and safely under very controlled conditions. Besides traditional production systems, such as bacteria, yeasts, insects and mammal cell lines, plants can now be used to produce eukaryotic recombinant proteins, especially therapeutic ones. Their advantages as hosts for protein production in...

Human butyrylcholinesterase is a performant stoichiometric bioscavenger of organophosphorous nerve agents. It is either isolated from outdated plasma or functionally expressed in eukaryotic systems. Here, we report the production of active human butyrylcholinesterase in a prokaryotic system after optimization of the primary sequence through the Protein Repair One Stop Shop process, a structure-...