Plural unit selection and fusion is a successful method for concatenative synthesis. Yet its unit fusion algorithm is simple and requires improvement. Previous research on unit fusion is mainly involved in boundary smoothing and not quite suitable for the application mentioned above. Therefore, a high-quality unit fusion method is proposed in this paper. More accurate pitch frame alignment and ...

Image fusion is an important process in the medical image diagnostics methods. Fusing images by obtaining information from different source and types of images(modals) called multi-modal fusion. This paper implements effective fast spatial domain based using moving frame decomposition (MFDF)method. Images two modalities are taken decomposed to texture approximation components. Weight mapping st...

As acute nonlymphocytic leukemia (ANLL) with inv(16) (p13q22) or t(16;16)(p13;q22) has been shown to result from the fusion of transcription factor subunit core binding factor (CBFB) to a myosin heavy chain (MYH11), we sought to design methods to detect this rearrangement using reverse transcriptase-polymerase chain reaction (RT-PCR). In all of 27 inv(16)(p13q22) and four t(16;16)(p13;q22) case...

We adapted a fusion polymerase chain reaction (PCR) strategy to synthesize gene disruption alleles of any sequenced yeast gene of interest. The first step of the construction is to amplify sequences flanking the reading frame we want to disrupt and to amplify the selectable marker sequence. Then we fuse the upstream fragment to the marker sequence by fusion PCR, isolate this product and fuse it...

In real applications, how to measure the uncertain degree of sensor reports before applying sensor data fusion is a big challenge. In this paper, in the frame of Dempster-Shafer evidence theory, a weighted belief entropy based on Deng entropy is proposed to quantify the uncertainty of uncertain information. The weight of the proposed belief entropy is based on the relative scale of a propositio...

To identify Rns-regulated genes, a maltose binding protein (MBP)-Rns fusion protein was used to isolate DNA fragments from enterotoxigenic Escherichia coli genomic DNA that carry Rns binding sites. In vivo transcription fusion analysis shows that Rns positively regulates the expression of the open reading frame of yiiS, which lies immediately downstream of one MBP-Rns-bound fragment.

The flagellin type III secretion pathway of Bacillus halodurans BhFC01 (Deltahag) was modified by the inactivation of fliD. An in-frame flagellin gene fusion polypeptide construct was expressed, and the heterologous peptides were secreted as flagellin fusion monomers. The stability of the secreted monomers was significantly enhanced through gene-targeted inactivation of extracellular proteases.

Fusion of biological membranes is a fundamental process in many cellular functions, including the intracellular delivery of lipids, proteins, and metabolites by transport vesicles, endocytosis, exocytosis, cell division, cell-cell fusion, fertilization, and the invasion of cells by enveloped viruses. These complex fusion processes involve proteins that bring the fusing membranes into close prox...

The CBP gene at 16p13 fuses to MOZ and MLL as a result of the t(8;16)(p11;p13) in acute (myelo)monocytic leukemias (AML M4/M5) and the t(11;16)(q23;p13) in treatment-related AML, respectively. We show here that a novel t(10;16)(q22;p13) in a childhood AML M5a leads to a MORF-CBP chimera. RT-PCR using MORF forward and CBP reverse primers amplified a MORF-CBP fusion in which nucleotide 3103 of MO...