In this work, proteins in extracellular polymeric substances extracted from anaerobic, anoxic and aerobic sludges of wastewater treatment plant (WWTP) were analyzed to probe their origins and functions. Extracellular proteins in WWTP sludges were identified using shotgun proteomics, and 130, 108 and 114 proteins in anaerobic, anoxic and aerobic samples were classified, respectively. Most protei...

Pannexin1 proteins form communication channels at the cell plasma membrane surface, which allow transfer of small molecules and ions between intracellular compartment extracellular environment. In this way, pannexin1 play an important role in various cellular processes diseases. Indeed, a plethora human pathologies is associated with activation channels. The present paper reviews summarizes str...

Choline and choline metabolites are essential for all cellular functions. They have also been reported to be crucial neural development. In this work, we studied the functional characteristics of uptake system in human stem cells (hNSCs). Additionally, investigated effect extracellular inhibition on activities hNSCs. We found that mRNAs proteins transporter-like protein 1 (CTL1) CTL2 were expre...

Proteomic mapping of cell surfaces is an invaluable tool for drug development and clinical diagnostics. This work describes a new ‘snapshot imprinting’ method designed to obtain proteomic maps surfaces, with the aim identifying surface markers epitopes diagnostic therapeutic applications. The analysis two cancer lines, HN5 MDA-MB-468, described herein as proof concept, along selective targeting...

Extracellular vesicles (EVs) play important roles in the diagnosis and treatment of diseases because its lavish bioinformatic molecular contents. However, how to separate plentiful extracellular a short time with high consistency is still problem. Here, we’d like introduce an automated capturing machine named EVenrich (Extracellular Vesicles Enrichment) that helps realize EVs biosamples high-th...