The research and development of extracorporeal bioartificial liver is gaining pace in recent years with the introduction of a myriad of optimally designed bioreactors with the ability to maintain long-term viability and liver-specific functions of hepatocytes. The design considerations for bioartificial liver are not trivial; it needs to consider factors such as the types of cell to be cultured...

This paper presents an experimental study of three bioreactor configurations. The bioreactor is intended to be used for the development of tissue-engineered heart valve substitutes. Therefore it must be able to reproduce physiological flow and pressure waveforms accurately. A detailed analysis of three bioreactor arrangements is presented using mathematical models based on the windkessel (WK) a...

The purpose of this study was to combine a three-dimensional NMR-compatible bioreactor with hyperpolarized (13)C NMR spectroscopy in order to probe cellular metabolism in real time. JM1 (immortalized rat hepatoma) cells were cultured in a three-dimensional NMR-compatible fluidized bioreactor. (31)P spectra were acquired before and after each injection of hyperpolarized [1-(13)C] pyruvate and su...

Limitations to nutrient transport provide a challenge to the development of 3D tissue-engineered constructs. A heterogeneous distribution of viable cells and functional matrix within the developing tissue is a common consequence. In the present study, a bioreactor was developed to perfuse fluid through cylindrical agarose constructs. The transport and distribution of dextran molecules (FD-4, FD...

Bioreactor landfills are operated to enhance refuse decomposition, gas production, and waste stabilization. The major aspect of bioreactor landfill operation is the recirculation of collected leachate back through the refuse mass. Due to the presence of additional leachate and accelerated decomposition, the dynamic characteristics of MSW in bioreactor landfills are expected to change. Assessmen...

Anaerobic oxidation of methane (AOM) in marine sediments is an important global methane sink, but the physiological characteristics of AOM-associated microorganisms remain poorly understood. Here we report the cultivation of an AOM microbial community from deep-sea methane-seep sediment using a continuous-flow bioreactor with polyurethane sponges, called the down-flow hanging sponge (DHS) biore...

the gf677 (prunus amygdalus×prunus persica) is a peach rootstock tolerant to fe deficiency .therefore, mass propagation of gf677 rootstock is recommended by bioreactor. on the other hand, plant micro-propagation using bioreactors can decrease the cost of production and makes it an economical method. in this research, the possibility of using bioreactor and optimization of the tissue culture pro...

The long-term stability of liver cell functions is a major challenge when studying hepatic drug transport, metabolism, and toxicity in vitro. The aim of the present study was to investigate organic anion-transporting polypeptide (OATP) 1B1 and CYP3A4 activities in fresh primary human hepatocytes and differentiated cryopreserved HepaRG cells when cultured in a three-dimensional (3D) bioreactor s...

The present study aimed at developing a strategy to improve the volumetric production of PHAs by Pseudomonas fluorescens S48 using waste frying oil (WFO) as the sole carbon source. For this purpose, several cultivations were set up to steadily improve nutrients supply to attain high cell density and high biopolymer productivity. The production of PHAs was examined in a 14 L bioreactor as one-st...

Title of Document: TUBULAR PERFUSION SYSTEM BIOREACTOR FOR THE DYNAMIC CULTURE OF HUMAN MESENCHYMAL STEM CELLS Andrew Bryan Yeatts, Doctor of Philosophy, 2012 Directed By: Associate Professor John P. Fisher, Fischell Department of Bioengineering In vitro culture techniques must be improved in order to increase the feasibility of cell based tissue engineering strategies. Limitations of current t...