نتایج جستجو برای: fission yeast

تعداد نتایج: 98162  

Journal: :Journal of Visualized Experiments 2016

Journal: :Nature Reviews Molecular Cell Biology 2010

Journal: :The Journal of Cell Biology 1989
T G Chappell G Warren

A membrane-associated galactosyltransferase has been purified to homogeneity from the fission yeast, Schizosaccharomyces pombe. The enzyme has a molecular weight of 61,000 and is capable of transfering galactose from UDP-galactose (UDP-Gal) to a variety of mannose-based acceptors to form an alpha-1,2 galactosyl mannoside linkage. Immunofluorescence localization of the protein is consistent with...

Journal: :Frontiers in bioscience : a journal and virtual library 1999
M N Boddy P Russell

The eukaryotic cell cycle comprises two critical phases, DNA replication (S phase) and the subsequent distribution of an equivalent genome to each of two daughter cells at mitosis (M phase). A signal transduction cascade called the replication checkpoint has evolved to ensure that M phase does not occur prior to the completion of S phase. The mitotic regulators targeted by this checkpoint have ...

Journal: :Trends in microbiology 2001
B Feierbach F Chang

The fission yeast Schizosaccharomyces pombe provides a genetic model system for the study of cytokinesis. As in many eukaryotes, cell division in the fission yeast requires an actin-myosin-based contractile ring. Numerous components of the contractile ring that function in ring assembly, positioning and contraction have been characterized. Many of these proteins are evolutionarily conserved, su...

Journal: :Cell cycle 2010
Mario Spirek Zsigmond Benko Martina Carnecka Cornelia Rumpf Lubos Cipak Monika Batova Ivana Marova Miyoung Nam Dong-Uk Kim Han-Oh Park Jacqueline Hayles Kwang-Lae Hoe Paul Nurse Juraj Gregan

The fission yeast Schizosaccharomyces pombe is a model organism used widely to study various aspects of eukaryotic biology. A collection of heterozygous diploid strains containing individual deletions in nearly all S. pombe genes has been created using a PCR based strategy. However, deletion of some genes has not been possible using this methodology. Here we use an efficient knockout strategy b...

Journal: :Genetics 2003
Gerald R Smith Michael N Boddy Paul Shanahan Paul Russell

Most models of homologous recombination invoke cleavage of Holliday junctions to explain crossing over. The Mus81.Eme1 endonuclease from fission yeast and humans cleaves Holliday junctions and other branched DNA structures, leaving its physiological substrate uncertain. We report here that Schizosaccharomyces pombe mus81 mutants have normal or elevated frequencies of gene conversion but 20- to ...

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