Present study was carried out among the elderly people (60+ years of age) from August 2005 to July 2006 in Kathmandu Valley to assess the prevalence of intestinal parasitosis in them. Stool samples were collected from 235 elderly people (122 from government elderly home, 66 from private elderly home and 47 from the households in a rural community). The samples were examined by formal ether sedi...

OBJECTIVE To find the prevalence of human intestinal pathogenic parasites. SETTING The study was conducted in Konkor, Gadap, District East, Karachi. METHODS After taking the verbal consent, all the villagers, selected by area sampling technique were interviewed by a structured questionnaire and then each person in the house was provided with a clean, plastic container to collect the stool s...

Vacuoles were isolated via protoplasts from the leaves of a halophyte Atriplex gmelini C.A.Mey., grown in culture solution supplemented with 250 millimolar NaCl. Lysis of the protoplasts was induced by lowering the medium osmolarity (1.2 to 1.0 molar sorbitol) and adding a detergent, a synthesized cholate derivative, 3-([3-cholamidopropyl] dimethylammonio)-1-propanesulfonate at a concentration ...

BACKGROUND This is the first work done on cryptosporidiosis among the children in Taiz, Yemen. METHODS A number of 712 samples were collected from children of different ages (ranging from 1 month to 12 years) from Dec 2006 to Aug 2007. The collected samples were examined by Sheather's sugar floatation and Modified Ziehl- Neelsen stain as well as ELISA methods. The test results were statistica...

This cross sectional study aims at determining the effect of gastrointestinal (GI) parasitism on haematological and biochemical parameters in goat. From Jaipur District of Rajasthan, 39 goats of either sex were screened by Direct Smear Method and simple floatation technique for the presence of helminthes eggs in their faeces. The goat found positive for eggs of helminthes eggs were again subjec...

Functional plasma membranes from the filamentous fungus Penicillium chrysogenum have been isolated with the objective of studying transport processes. The isolation procedure consists of three steps, namely homogenization of cells with a Braun MSK homogenizer, followed by Percoll gradient centrifugation and floatation of membranes in a three-step Nycodenz gradient. This method can be applied to...

Detergent resistant membranes (DRMs) are the site of assembly for a variety of viruses. Here, we make use of Sendai virus mutant proteins that are not packaged into virus particles to determine the involvement of this assembly for the virus particle production. We found that, in the context of an infection, (1) all the Sendai virus proteins associated in part with DRMs, (2) mutant HN and M prot...

Four-dimensional imaging, which indicates imaging in three spatial dimensions as a function of time, provides useful evidence to investigate the interactions of rising bubbles. However, this has been largely unexplored for microbubbles, mostly due to problems associated with strong light scattering and shallow depth of field in optical imaging. Here, tracking x-ray microtomography is used to vi...

BACKGROUND Intestinal parasites are very common in developing countries including Nigeria. There are diverse ways of their transmission; the study attempts to determine the level of intestinal parasitic contamination on vegetables sold in Jos. METHODS Sample of 200 each of Tomatoes (Lycopersium sativus), Letus (Loctus satival) Carrot (Davcus carota L) Cabbage (Brassica Denceal) and Green leaf...

This study was geared towards investigating the prevalence of gastrointestinal parasites of hunting dogs in Maiduguri, Borno state, Nigeria. A total of 250 feacal samples were collected per rectum. The parasites were determined using formol ether and floatation concentration methods. An overall prevalence of 38% for gastrointestinal infection was recorded. The prevalence was higher in young 39(...