1026 Background: Predictive markers of response to chemotherapy are lacking in breast cancer patients. Preclinical data suggest that breast cancer cells that loose expression of Foxp3 gene develop resistance to anthracyclines but not to taxane. However, only few conflicting clinical data are available. Using Foxp3 labelling we tested the hypothesis that adjuvant chemotherapy incorporating docet...

Foxp3 is a master regulator of CD4(+)CD25(+) regulatory T-cell (Treg) function and is also a suppressor of SKP2 and HER2/ErbB2. There are an increasing number of reports describing the functions of Foxp3 in cell types other than Tregs. In this context, we evaluated the functions of Foxp3 in ovalbumin- and cockroach-induced asthma models. Foxp3-EGFP-expressing adenovirus or EGFP control adenovir...

The X-linked FOXP3 gene is a member of the forkhead box/winged helix family. As a transcription factor, FOXP3 plays an important role in the immune system by acting as a master regulator of transcription for the development and function of regulatory T cells. Additionally, its tumor suppressive activity has been observed during tumor initiation [1]. MicroRNA (miR) plays a role in RNA silencing ...

PAUL E. OLSEN1, HANS-DIETER SUES2, and MARK A. NORELL3 1Lamont-Doherty Earth Observatory, Columbia University, Palisades, New York 10964; 2Department of Palaeobiology, Royal Ontario Museum, 100 Queen’s Park, Toronto, Ontario, Canada M5S 2C6 and Department of Zoology, University of Toronto, Toronto, Ontario, Canada M5S 3G5; 3Division of Paleontology, American Museum of Natural History, Central P...

there are limited clinical investigations identifying the percentage of t helper 1 (th1) and t regulatory (treg) cells in stable as well as rejected kidney allografts, a concept which needs to be more studied. the aim of our study was to compare the percentage of cd4+ ifn-γ+ cells, the number of ifn-γ secreting cells and the amount of foxp3 expression in patients with or without stable graft fu...

Forkhead Box P3 (FOXP3) is a member of the forkhead/winged helix family of the transcription factors and plays an important role not only as a master gene in T-regulatory cells, but also as a tumor suppressor. In this study, we identified lymphocyte-specific protein tyrosine kinase (LCK), which correlates with cancer malignancy, as a binding partner of FOXP3. FOXP3 downregulated LCK-induced MMP...

BACKGROUND Naturally occurring thymus derived regulatory T cells (Tregs) are central in the maintenance of self-tolerance. The transcription factor FOXP3 is crucial for the suppressive activity of Tregs and is considered the most specific marker for this population. However, human non regulatory T cells upregulate FOXP3 transiently upon activation which calls for other means to identify the Tre...

CD4(+)CD25(+)Foxp3(+) regulatory T (Treg) cells are able to inhibit proliferation and cytokine production in effector T-cells and play a major role in immune responses and prevention of autoimmune disease. A master regulator of Treg cell development and function is the transcription factor Foxp3. Several cytokines, such as TGF-β and IL-2, are known to regulate Foxp3 expression as well as methyl...

BACKGROUND The FOXP3 gene is the master regulator for T regulatory cells and is under tight DNA methylation control at the Treg specific demethylated region (TSDR) in its first intron. This said, methylation of its promoter region, the significance of which is unknown, has also been associated with various immune-related disease states such as asthma, food allergy, auto-immunity and cancer. Her...

Materials and methods The single nucleotide polymorphism (SNP) at position 543 of exon 5 of FOXP3 was investigated in 58 caucasian children patients with KS (F:30, M:28), recruited at our Paediatric Reumatology Unit. In 6 patients we could evaluate the percentage of circulating CD4+ T cells co-expressing CD25 and FOXP3 by flow cytometry, mRNA transcripts of FOXP3 in PBMCs by Real Time PCR (with...