Maintaining iron homeostasis is a necessity for all living organisms, as free iron augments the generation of reactive oxygen species like superoxide anions, at the risk of subsequent lethal cellular damage. The iron-responsive regulator Fur controls iron metabolism in many bacteria, including the important human pathogen Helicobacter pylori, and thus is directly or indirectly involved in regul...

A series of bidentate hydroxypyridinone iron chelators that have therapeutic potential as oral iron chelators, have been studied systematically to determine which properties are the most critical for the mobilization of hepatocyte iron. The relationship between lipid solubility of the free and complexed forms of each chelator and hepatocyte iron release has been investigated as well as the cont...

The majority of iron for essential mammalian biological activities such as erythropoiesis is thought to be reutilized from cellular hemoproteins. Here, we generated mice lacking functional heme oxygenase 1 (Hmox1; EC 1.14.99.3), which catabolizes heme to biliverdin, carbon monoxide, and free iron, to assess its participation in iron homeostasis. Hmox1-deficient adult mice developed an anemia as...

background: metals such as iron, zinc, and copper are critical and necessary for the survival of all living organisms, whereas xenobiotic metals such as lead, cadmium, mercury, and arsenic have no known biologic role. any metals in high doses can have toxic effects. the aim of this work was to evaluate the hematological changes induced by lead as a toxic metal and characterize the potential eff...

βThalassemia is an inherited genetic disorder which is caused by different kinds of mutations in the HBB gene in chromosome 11. Due to several types of mutation in βgene, globin chains cannot synthesise completely and free α-globin is highly unstable and readily precipitates bound heme and iron. In βthalassemia these precipitated iron, repeated blood transfusion and increased gastrointestinal i...

Rat liver and intestinal microsomes were exposed to various free radical generating systems and their effect were assessed by studying different parameters such as formation of malonaldehyde (MDA) and conjugated diene, arachidonic acid depletion and alteration in protein thiol groups and tocopherol levels. These studies revealed that liver being highly vulnerable tissue showed all the effects o...

conjugation of monoclonal antibodies to super paramagnetic nanoparticles is an effective method for cancer diagnosis and treatment. in this study the humanized anti her2/neu monoclonal antibody- herceptin- was conjugated to super paramagnetic iron oxide (spio) nanoparticles using edc method. the concentration of the conjugated antibodies was measured by bradford assay. the antibody-nanoparticle...