The calculated freezing point depression of freshly excised boiled mammalian tissue is approximately the same as that of plasma. The boiling procedure was chosen to eliminate the influence of metabolism on the level of the freezing point depression. Problems created by the boiling, such as equilibrium between tissue and diluent, change in activity coefficient by dilution, and loss of CO(2) cont...

It is shown that a fluid near a topographically patterned wall exhibits crystallization below the bulk freezing point (so-called precrystallization). In detail, a periodic array of fixed hard spheres is considered as a wall pattern. The actual type of the pattern corresponds to a face-centred-cubic (fcc) lattice cut along the (111), (100) or (110) orientation, a hexagonal-close-packed (hcp) sol...

Although the biochemical correlates of freeze tolerance in insects are becoming well-known, the process of ice formation in vivo is subject to speculation. We used synchrotron x-rays to directly visualise real-time ice formation at 3.3 Hz in intact insects. We observed freezing in diapausing 3(rd) instar larvae of Chymomyza amoena (Diptera: Drosophilidae), which survive freezing if it occurs ab...

Rapid Freezing Prototyping (RFP) with water is a novel solid freeform fabrication technique that can generate three-dimensional ice objects by depositing and rapidly freezing water layer by layer. The support where necessary is made of brine whose. freezing point is lower than. pure water. After building the part, the support can be removed by utilizing the melting temperature difference betwee...

abstract plants growth and development and physiological activities occur in a certain air temperature range. spit of this fact that zero temperature named as a freezing temperature, in agriculture meteorology, freezing happen in lower temperature which is different for plants that lead to their tissues damage. early autumn freezing cause damage to harvesting of cotton and sugar beet and affect...

The serious health risks posed by bone fractures create a growing need for accurately diagnosing fracture risk. The Reference Point Indentation instrument (RPI) directly measures key mechanical properties in vivo to assess bone fracturability. There is a wealth of information that could be obtained from measuring cryopreserved bone samples with the RPI. Since it is unknown how freezing affects ...

OBJECTIVES To document and characterize freezing temperatures in the Indonesian vaccine cold chain and to evaluate the feasibility of changes designed to reduce the occurrence of freezing. METHODS Data loggers were used to measure temperatures of shipments of hepatitis B vaccine from manufacturer to point of use. Baseline conditions and three intervention phases were monitored. During each of...

To a very large extent progress in the field of invertebrate physiology is a measure of progress in the technique of manipulating small structures and analysing small samples. Within this field the study of osmotic regulation is no exception. Krogh (1938) has summarized the known methods of measuring osmotic pressure, applicable to quantities of fluid of the order of O-I-I-O cu.mm. The freezing...

Monodisperse granular flows often develop regions with hexagonal close packing of particles. We investigate this effect in a system of inelastic hard spheres driven from below by a "thermal" plate. Molecular dynamics simulations show, in a wide range of parameters, a close-packed cluster supported by a low-density region. Surprisingly, the steady-state density profile, including the close-packe...

Metabolic activity was measured in the laboratory at temperatures between 5 and -20 degrees C on the basis of incorporation of (14)C-labeled acetate into lipids by samples of a natural population of bacteria from Siberian permafrost (permanently frozen soil). Incorporation followed a sigmoidal pattern similar to growth curves. At all temperatures, the log phase was followed, within 200 to 350 d...