Scientific and medical development simulates reality reflects its image as a natural inevitability necessity of life. However, the misconduct or abuse right that some biologists who specialize in this field may commit, what is practiced by specialized doctors during their application technique human reproductive fertilization, such negligently deliberately manipulating cells destroying them, wi...

the many and different endeavors which aim at an explanation of living phenomena in terms of molecules and their properties. The present work by 25 outstanding scientists in molecular biology points up the tremendous upsurge of research activity on the molecular level. The book contains 20 examples of this research, all written by investigators deeply engaged in it. The chapters illustrate the ...

Fertilized rabbit ova at the 2-blastomere stage kept in rabbit serum were stored at low temperatures for various lengths of time. They were then cultured at 38 degrees C. for about 24 hours to determine their viability. A number of the viable ova were finally transplanted into recipient does. It was found that rapid cooling of ova to 5 degrees or to 0 degrees C. was more harmful to the subseque...

In this study, we investigated the role of ovalbumin (OVA), ovotransferrin (OVT), and lysozyme (LYZ) as major proteins involved in heat-induced gelation egg white. OVT LYZ deficient white (OLdEW) solutions were prepared from raw (REW) subjected to heat treatment at 64 °C centrifugation. OLdEW had almost same native-OVA protein REW, confirmed by gel electrophoresis, differential scanning calorim...

The adjuvant capacity of a novel vaccine vector "Gantrez-nanoparticles" (NP) towards coated or encapsulated ovalbumin (OVA) was investigated. OVA nanoparticles were prepared by a solvent displacement method previously described. The protein was incorporated during the manufacturing process (OVA-encapsulated nanoparticles) or after the preparation (OVA-coated nanoparticles). The mean size of the...

20 Background: Allergen-specific immunotherapy has been anticipated to be a 21 disease-modifying therapy for food allergies. We previously reported that CD8 + 22 regulatory T cells may prevent antigen-sensitized mice from developing allergic 23 diarrhea. Because oligomannose-coated liposomes (OML) have been shown to induce 24 MHC class I-restricted CD8 + T cell responses, we analyzed the adjuva...

This study aimed to present a mouse model of ovalbumin (OVA) induced allergic diarrhea under a sub-barrier system and investigate the development of gut microbiota in this model. Male BALB/c mice were systemically sensitized with OVA or sham-sensitized with saline, and followed by oral OVA intubation, leading to OVA-specific acute diarrhea. Compared with sham-sensitized mice, sera OVA-specific ...

We evaluated CD8(+) T cell responses against the dominant CTL epitope, OVA(257-264), expressed by an acute (Listeria monocytogenes (LM) OVA) vs a chronic pathogen (Mycobacterium bovis bacillus Calmette-Guérin (BCG) OVA) to reveal the influence on CD8(+) T cell memory and consequent protection against a challenge with OVA-expressing tumor cells. Infection with lower doses of both pathogens resul...

Effects of a Kampo (Japanese herbal) medicine "shoseiryuto (SST, xiao-qing-long-tang in Chinese)", which has been used for the treatment of allergic bronchial asthma clinically, were examined on ovalbumin (OVA)-sensitized allergic airway inflammation model (i.e., bronchial asthma) in a mouse. When SST was orally administered at 0.5 g kg(-1) day(-1) from day 1 to 6 after OVA inhalation, SST redu...

BACKGROUND Several methods have been reported to induce asthmatic reactions in mice but few studies have compared their efficiency. We evaluated the efficiency of the protocols frequently used in the literature. METHODS BALB/c mice were sensitized to ovalbumin (OVA) by intraperitoneal injection; 1] Once a week for two weeks using OVA with alum (IPOA-2) or without (IPO-2), and provocation on d...