The current cervical artificial insemination (CAI) procedure, involving deposition of excessive sperm numbers, is uneconomical for pig industry. The most obvious alternative requires uterine deposition in combination with fixed-time AI, which would reduce the number of sperm required per pregnant sow, thus allowing the best use of valuable boars and, ultimately, the commercial integration of fr...

OBJECTIVE To achieve maximum post-thaw survival of frozen embryos. DESIGN Historical controlled study. SETTING Hospital-based fertility center. PATIENT(S) One hundred forty-five patients whose embryos were frozen and thawed according to the standard method, and 56 patients whose embryos were frozen and thawed according to a modified method. INTERVENTION(S) Modifications were made to the...

BACKGROUND Embryo culture media used for IVF treatment might affect fetal growth and thus birthweight of the newborns. METHODS A retrospective study was conducted in South China using data from 2370 singleton neonates born after IVF/ICSI between 2009 and 2012. Two culture media, i.e., either Vitrolife or SAGE were used as embryo culture media during the study period. Neonates' birthweights we...

OBJECTIVE This study was performed to compare the efficiency of slow freezing and vitrification based on survival, development to blastocysts, and cell numbers of blastocysts. Changes in embryonic gene expression in fresh and frozen-thawed embryos were also examined. METHODS Eight-cell stage embryos were collected from superovulated female BDF1 mice. The collected embryos were randomly divide...

The present study was performed to compare the expression of sperm proteins, i.e. triosephosphate isomerase (TPI) and acrosin binding protein (ACRBP) seminal plasma glutathione peroxidase 5 (GPX5) fibronectin 1 (FN1), in boar semen with good, moderate poor freezability. conducted by determining contents 32 samples 38 semen. ejaculated divided into two portions: first portion centrifuged separat...

background: the basis of spermatogenesis is the spermatogonial stem cells (sscs). the concentration of sscs is very small. however, a system that supports the proliferation and maintenance of sscs in vitro could be used to preserve and expand sscs numbers as well as increase success in transplantation. it is a new avenue to restore spermatogenesis in azoospermia subjects. objective: proliferati...

Introduction While conception rates following cervical insemination of synchronised ewes with fresh semen are generally around 60-70%, conception rates with frozen-thawed semen are unacceptably low (<20% generally). These poor conception rates following cervical insemination with frozen-thawed semen appear to stem from two inter-related factors which are additive in their negative impacts: (a) ...

background: gnrh agonist and antagonist were developed to control the premature release of lh surge. there is some difference between two protocols. objective: we compared the outcome of frozen-thawed embryo transfer in infertile women who used gnrh agonist or antagonist protocol for previous coh cycle and evaluation of any adverse effect of gnrh antagonist on oocyte and embryo. materials and m...