this study investigated the inhibitory effects of bacillus licheniformis on g1 and g2 aflatoxins in culture media and kilka fish meal. two concentrations of aflatoxins (8 and 12 ppb for g1 and g2) and (2 and 4 ml from aflatoxins standard per 50g of fish meal) were used in culture media and kilka fish meal respectively. the doses of bacillus were 3 and 4% for this experiment. changes of aflatoxi...

AIM To construct synthetic antigens using the epitope of human brain acetylcholinesterase (hbAChE) for induction and detection of the specific antibody against the epitope, and to analyse the immunogenicity of the antibody. METHODS The epitope (RTVLVSMNYR, amino acids 143-152) of hbAChE was chemically synthesized, coupled with the carrier protein keyhole limpet hemocyanin (KLH) to construct a...

Objective(s):Brucellosis is a well-known domestic animal infectious disease, which is caused by Brucella bacterium. GroEL antigen increases Brucella survival and is one of the major antigens that stimulates the immune system. Hence, the objective of the present study was cloning and bioinformatics analysis of GroEL gene. Materials and Methods: The full-length open reading frame of this gene was...

The livestock sector in Pakistan occupies a unique position in the national agenda of economic development. The current study was conducted to evaluate a heat stress mitigation strategy through different feeding times on the performance i.e. milk yield, milk composition and physiochemical properties of milk in dairy cattle during the summer season. Twenty Holstein Friesian cows (HF) were random...

in our search for new anticancer medicinal plants, the antiproliferative activity of the methanol extract of daphne mucronata (thymelaeaceae) was evaluated using human myelogenous leukemia k562 cells. the cells responded to plant treatments in a dose dependent manner and the ic50 of the crude extract (equivalent to 1 g of plant leaves powder per ml) and the purified active component were found ...

OprF, the major outer membrane (OM) protein of Pseudomonas aeruginosa, has been proposed to be comprised of a series of beta-strands separated by periplasmic or surface-exposed loop regions. In this study, a simple malarial epitope was used to demonstrate that OprF can be used as an expression vector to present foreign peptide sequences, namely, the 4-amino-acid (aa) repeating epitope (Asn-Ala-...

Pseudoexfoliation syndrome (PEXS) and glaucoma (PEXG) are assumed to be caused by a generalized elastosis leading the accumulation of PEX material in ocular as well extraocular tissues. The exact pathophysiology PEXS is still elusive. PEXG, most common type secondary open-angle (OAG), characterized large peaks intraocular pressure (IOP) with progressive loss visual field. Agonistic autoantibodi...