This paper presents in vitro microvascular network formation within 3D gel scaffolds made from different concentrations of type-I collagen, fibrin, or a mixture of collagen and fibrin, using a simple microfluidic platform. Initially, microvascular network formation of human umbilical vein endothelial cells was examined using live time-lapse confocal microscopy every 90 min from 3 h to 12 h afte...

Much of the complex medical physics work requires radiation dose delivery, which dosimeters to accurately measure three-dimensional distribution with good spatial resolution. MAGIC-f polymer gel is one emerging new widely used in research. The purpose this study was present an overview dosimetry, using gel, including its composition, manufacture, imaging, calibration, and application In review,...

We were able to produce the first ever meltderived bioactive glass scaffold that met the requirement of an ideal bone scaffold by using the gel-cast foaming process and a new glass composition. However, initially a small fraction of sodium potassium sulfate crystals formed during processing. The purpose of this work was to study and prohibit the growth of those crystals, which was the result of...

Here, we describe an affinity isolation protocol. It uses cryomilled yeast cell powder for producing cell extracts and antibody-conjugated paramagnetic beads for affinity capture. Guidelines for determining the optimal extraction solvent composition are provided. Captured proteins are eluted in a denaturing solvent (sodium dodecyl sulfate polyacrylamide gel electrophoresis sample buffer) for ge...

Acyl phosphatase has been purified 25,000-fold from bovine brain. The enzyme is more than 97% pure by acrylamide gel disc electrophoresis. The calculated molecular weight from gel filtration measurements on Sephadex G-75 was 12,100; the value calculated from the amino acid composition was 8,732. Independent determinations of free -SH groups and tyrosine content were in agreement with the amino ...

The structures of large O-glycosidically linked oligosaccharides derived from swine trachea mucin glycoprotein were established. Reduced oligosaccharides released by treatment with alkaline borohydride were separated by gel filtration on Bio-Gel P-6 and the neutral oligosaccharides were isolated by chromatography on DEAE-cellulose. Eight oligosaccharides (DIa to BII), ranging in length from 8 t...

The enzyme anthranilate-5-phosphoribosylpyrophosphate phosphoribosyltransferase from Serratia marcescens was purified to apparent homogeneity. The purification procedure included ammonium sulfate precipitation, DEAE-cellulose chromatography, Sephadex gel filtration and hydroxyapatite chromatography. The molecular weight of the native protein as determined on a calibrated Sephadex G-200 column w...