Genotoxic effects of EMS have been assessed in fish, A. testudineus, using widely accepted cytogenetic protocols like chromosome aberrations, nuclear anomalies in red blood cells and abnormal sperm head morphology. In addition, gel electrophoretic protein profiles and total protein contents in nine selected tissues were analysed for evaluating their utility as potential indicators of genotoxici...

The degradation of C.I. Direct red 80, a polyazo dye, was investigated using Bacillus firmus immobilized by entrapment in tubular polymeric gel. This bacterial strain was able to completely decolorize 50 mg/L of C.I. Direct red 80 under anoxic conditions within 12 h and also degrade the reaction intermediates (aromatic amines) during the subsequent 12 h under aerobic conditions. The tubular gel...

Plasma from hemodialysis patients evoked weak photon emissions (chemiluminescence) in a characteristic emission spectrum with a peak at 430 nm, attributed to attack by hydroxyl radicals generated from the iron-catalyzed breakdown of hydrogen peroxide (Fenton reaction), whereas plasma from normal healthy subjects showed a rather weak red chemiluminescence peak at around 680 nm, similar to that r...

BACKGROUND Antibody titration is difficult to standardize. We investigated whether a detailed, uniform procedure for antibody titration would reduce variation in both tube-based and gel card titres in an international study. METHODS Laboratories (n = 35) tested proficiency testing material provided by the College of American Pathologists each according to (i) their routine method; (ii) a deta...

To obtain the large quantities of the extracellular domain of the nerve growth factor receptor (NGF-R) necessary for structural analyses, we produced this protein in the baculovirus expression system. A cDNA coding for the extracellular domain of the human NGF-R was first introduced into transfer vector pVL941. Recombinant baculovirus was produced by cotransfecting Spodoptera frugiperda cells w...

Human erythrocyte membrane glycoproteins were solubilized and recovered in the aqueous phase after extraction of red cell ghosts with a mixture of chloroform and methanol. The major glycoprotein, the so-called MN glycoprotein, was prepared from this phase by gel filtration on Sepharose 4B columns in 6 M guanidine hydrochloride. By SDS-acrylamide gel electrophoresis the MN glycoproteins from the...

In this report, we successfully fabricated the colloidal crystal (CC) gel films of silica microparticles combined with a temperature-responsive biocompatible hydrogel poly(N-vinylcaprolactam) (VCL). When CC VCL film was prepared by filling precursor into void space between particles film, followed thermal polymerization, Bragg reflection peak red-shifted from 475 nm to 535 due change refractive...

In this work, α-Fe2O3 (hematite) nanoparticles were synthesized using Arabic gum (AG) as a biotemplate source by the sol-gel method. This method has many advantages such as low-cost, nontoxicity, simple work-up, high efficiency, compounds uniformity, and high efficiency. The α-Fe2O3 nanoparticles were characterized by Fourier transform infrared (FT-IR...

The extraction of nucleic acids with phenol and chloroform is the standard method to remove proteins and lipids from cellular digests. A major drawback to the use of these organic solvents is their caustic and toxic qualities. Phenol can cause severe burns while chloroform is hepatotoxic and carcinogenic. We describe in this paper the use of a special blood collection tube to isolate the offens...