This paper describes convenient preparations of heterogeneous multisectional polyacrylamide slab gels and the protocols that use these gels to measure protein-ligand binding constants [using bovine carbonic anhydrase B (CAB) as a model system]. Unlike procedures for affinity electrophoresis using tube gels, all binding information concerning protein-ligand interactions was encoded in a single m...

Calcium-enhanced protein recovered from black bullhead catfish was used to develop gels containing increasing amounts of potato starch (0-20 g/kg protein paste) and the effects of starch on functional, textural, and color properties were tested. Energy required to unfold protein groups was greater with the addition of 5 g starch/kg protein paste. Gels containing starch were harder, chewier, and...

The aim of our study was to identify factors affecting the in vitro release diclofenac sodium (DS) from hypromellose-based gels (HPMC).
 Materials and methods. Gels with HPMC liquids without were studied by viscosity-rotating viscometer method spin probe electron paramagnetic resonance spectroscopy. Rheograms used determine flow behavior apparent viscosity, EPR spectra rotational correlati...

Anchote is a tuber crop indigenous to Ethiopia. Starch hydration properties and important gel characteristics which include: color, rheological (at 2, 4, 6, 8, 10% starch:water w/w) texture evolution w/w), during 0 192 h storage 4 °C), of anchote starches isolated from four cultivars (Desta 01, Desta 24, white red) were evaluated compared with potato cassava (PS CS). The lightness (L*) whitenes...

Here, the application of the gel-forming carbohydrate polymer, gellan gum, for the electrophoresis and isolation of DNA is detailed. Gellan gun forms gels in the presence of divalent metal cations, and the gels can be converted back to a solution by the addition of a chelating agent such as EDTA. Also, gellan electrophoresis gels can be formed using diamines. These gels are reversible by increa...

The evaluation of proteins using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis is a common technique used by biochemistry and molecular biology researchers. For laboratories that perform daily analyses of proteins, the cost of commercially available polyacrylamide gels (~$10/gel) can be considerable over time. To mitigate this cost, some researchers prepare their...

Pulsed field gel electrophoresis (PFGE), the gold standard of molecular typing methods, has a major disadvantage of an unusually long electrophoretic time. From the original protocol of 6 days, it was modified to 3 days and subsequently to a single day. We describe the procedure of stacking five to six gels one on top of another in order to increase and maximize the output in a shorter time wit...

ketorolac tromethamine (kt) containing microemulsion-based gels (mbgs) have been formulated, based on the previous phase diagram studies, using a pharmaceutically acceptable surfactant (soybean lecithin; epikuron 200) and oil (isopropyl myristate; ipm) and the effect of formulation variables on the release profile of the drug from mbgs through intact guinea pig skin and various artificial membr...

monolithic aerogels of high molecular weight polyethylene (mw= 3x106- 6x106 g/mol) have been prepared by solvent extraction with supercritical carbon dioxide from thermoreversible gels prepared in decalin. these low density and highly porous aerogels present an apparent porosity up to 90%. the aerogel morphology observed by scanning electron microscopy (sem) is characterized by spherulitic stru...