objective: the objective of this study is to develop and assess targeted pamam-peg nanocarrier with anti-tag72 nanobody for t-bid gene coding construct delivery into the human colonic adenocarcinoma cells. materials and methods: nanobody (nb) coding sequence was subcloned into psj expression vector for large-scale production and then nb was purified by ni++ affinity chromatography. sds-page an...

Gene delivery is a powerful technique that often exploited in the treatment of several diseases.

In the evolution of AIE-based drug and gene delivery systems, light-up AIEgens start in real-time monitoring processes evaluation therapeutic responses drug/gene; The delicate structural design to form high-level carriers remarkably enhances drug/gene efficiency; Further, endowed with multifunctionalities, are synergized multimodality therapy achieve augmented efficiency(e282).

Gene therapy has undergone a remarkable development in the last 20 years. Particularly important advances have been made in the improvement of gene transfer and expression technology, with current efforts focusing on the design of safer and long-term gene expression vectors as well as systems possessing cell-type specificity for transgene delivery and regulat‐ ability of its expression by small...

Human gene therapy holds great promise in treating not only hereditary genetic disorders, but also disease states such as cancer and viral infections, and contingencies such as stroke or myocardial infarctions. It can be achieved by delivery of a correct gene into target cells with genetic deficiency or mutations, or by transfer of a therapeutic agent such as agents targeting a cancer-causing o...

It has been over 40 years since I started biomaterials research. This article is a short summary of past research in my laboratory.

Dendrimers have unique molecular architectures and properties that make them attractive materials for the development of nanomedicines. Key properties such as defined architecture and a high ratio of multivalent surface moieties to molecular volume also make these nanoscaled materials highly interesting for the development of synthetic (non-viral) vectors for therapeutic nucleic acids. Rational...

Gene therapy bases its rationale on the transfer of genetic components (genes or fragments thereof) into somatic cells, with the aim of preventing, correcting, or healing various types of disorders. After this introductory sentence you expect us to start telling you some of the marvellous achievements in setting up the tools that allow this transfer. However, before entering into the intricate ...

One approach to gene therapy for human cancer is transcatheter injection of DNA liposomes into tumor masses. T o determine the feasibility of selective delivery of recombinant genes by a catheter to the pulmonary vasculature in humans, a patient with melanoma received two treatments of H L A B 7 plasmid D N A complexed to cationic liposomes into a right posterior basal pulmonary artery associat...