objective(s): first, 10-bromodecanoic acid was covalently attached to all available surface primary amines of ppi g2 and g3 to increase their lipophilicity. in the subsequent step, ppis were conjugated to the alkylcarboxylate groups of alkylcarboxylate-ppi derivatives to increase the number of surface primary amines. physicochemical properties of modified ppis were determined. transfection expe...

Microglial activation is a central event in neurodegeneration. Novel technologies are sought for that specifically manipulate microglial function in order to delineate their role in onset and progression of neuropathologies. We investigated for the first time whether non-viral gene delivery based on polyethyleneglycol-polyethyleneimine conjugated to the monoclonal anti-CD11b antibody OX42 ("OX4...

Human turbinate mesenchymal stromal cells (hTMSCs) are novel stem cells derived from nasal inferior turbinate tissues. They are easy to isolate from the donated tissue after turbinectomy or conchotomy. In this study, we applied hTMSCs to a nonviral gene delivery system using polyethyleneimine (PEI) as a gene carrier; furthermore, the cytotoxicity and transfection efficiency of hTMSCs were evalu...

Stem-cell-derived hepatocyte transplantation is considered as a potential method for the therapy of acute and chronic liver failure. However, the low efficiency of differentiation into mature and functional hepatocytes remains a major challenge for clinical applications. By using polyethyleneimine-modified silica nanoparticles, this study develops a system for sustained delivery of growth facto...

gene therapy as a modern therapeutic approach has not yet advanced to a globally-approved therapeutic approach. lack of adequate reliable gene delivery system seems to be one of the major reasons from the pharmaceutical biotechnology point of view. main obstacles delaying successful application of human gene therapy are presented in this review. the unique advantages of non-biological gene carr...

the current methods for treatment of cancers are inadequate and more specific methods such as gene therapy are in progress. among different vehicles, cationic liposomes are frequently used for delivery of genetic material. this investigation aims to prepare and optimize dotap cationic liposomes containing an antisense oligonuclotide (asodn) against protein kinase c alpha in non-small cells lung...

application of therapeutic gene transfer in the treatment of genetic diseases is a notable progress but there are some disadvantages and limitations in it. the process of overcoming these barriers is a drastic change in gene delivery. recently, calcium phosphate nanoparticles alone, or in combination with viral and nonviral vectors, were found to have a positive effect on gene transfer especial...

BACKGROUND Many in vitro studies have demonstrated that silencing of cancerous genes by siRNAs is a potential therapeutic approach for blocking tumor growth. However, siRNAs are not cell type-selective, cannot specifically target tumor cells, and therefore have limited in vivo application for siRNA-mediated gene therapy. RESULTS In this study, we tested a functional RNA nanocomplex which excl...