The advent of the CRISPR/Cas9 system has transformed field human genome engineering and created new perspectives in development innovative cell therapies. However, absence a simple, fast efficient delivery method into primary cells been limiting progress CRISPR/Cas9-based Here, we describe an optimized protocol for iTOP-mediated various cells, including T induced pluripotent stem (hiPSCs), Jurk...

T he current kerfuffle around the use of CRISPR/Cas9 and other gene editing technologies in human germline research is the latest in a series of related controversies at the intersection of science, medicine, and ethics [1]. Soon after a prominent ad hoc group of scientists called for a moratorium on clinical applications of germline gene editing [2], a research group from China published an ar...

Gossypium hirsutum is an allotetraploid with a complex genome. Most genes have multiple copies that belong to At and Dt subgenomes. Sequence similarity is also very high between gene homologues. To efficiently achieve site/gene-specific mutation is quite needed. Due to its high efficiency and robustness, the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 system has exer...

Insect genome editing was first reported 1991 in Drosophila melanogaster but the technology used was not portable to other species. Not until the recent development of facile, engineered DNA endonuclease systems has gene editing become widely available to insect scientists. Most applications in insects to date have been technical in nature but this is rapidly changing. Functional genomics and g...

The advent of clustered regularly interspaced short palindromic repeat (CRISPR) has had a profound impact on plant biology, and crop improvement. In this review, we summarize the state-of-the-art development CRISPR technologies their applications in plants, from initial introduction random small indel (insertion or deletion) mutations at target genomic loci to precision editing such as base edi...

Epidermolytic palmoplantar keratoderma (EPPK) is a rare, autosomal dominantly inherited skin disorder. The disease often caused by heterozygous point mutations in the keratin 9 gene coding for type I intermediate filament (IF) protein (K9). integration of mutant K9 into IF cytoskeleton has dominant negative effect resulting fragility and massive thickening skin. aim this project was to correct ...

A comparison of the nucleotide sequences from genomic DNA and cDNA of the ndhB gene from maize chloroplasts shows that the ndhB transcript is edited by C-to-U transitions at six positions which appear to exist as editing sites also in the chloroplast ndhB genes from rice and tobacco but not from liverwort. In order to identify possible sequence determinants necessary for editing, the sequences ...

Genome editing is highly useful for crop improvement. The method of expressing genome-editing enzymes using a transient expression system in Agrobacterium, called agrobacterial mutagenesis, shortcut used technology to improve elite varieties vegetatively propagated crops, including potato. However, with this method, edited individuals cannot be selected. regeneration-promoting genes can result ...

In the past year, there has been more CRISPR news than awarding of Nobel Prize in Chemistry to Jennifer Doudna and Emmanuelle Charpentier. Gene editing continued its forward momentum major ways, scientists are beginning see results.

The case for rapid use of gene-editing technology The rise of the platform economy Rethinking society's active orientation Data-driven science policy Super-muscly pigs: Trading ethics for efficiency