Current disease association studies are routinely conducted on a genome-wide scale, testing hundreds of thousands or millions of genetic markers. Besides detecting marginal associations of individual markers with the disease, it is also of interest to identify gene-gene and gene-environment interactions, which confer susceptibility to the disease risk. The astronomical number of possible combin...

Basics of Linkage and Gene Mapping ......................................................................45 Linkage....................................................................................................................45 Linkage disequilibrium ............................................................................................47 Mapping functions ..............................

Estimating differences in gene expression among alleles is of high interest for many areas in biology and medicine. Here, we present a user-friendly software tool, Allim, to estimate allele-specific gene expression. Because mapping bias is a major problem for reliable estimates of allele-specific gene expression using RNA-seq, Allim combines two different strategies to account for the mapping b...

Fluorescence in situ hybridization (FISH) resolution has advanced because newer techniques use increasingly decondensed chromatin. FISH cannot analyze restriction enzyme cutting sites due to limitations of the hybridization and detection technologies. The RecA-assisted restriction endonuclease (RARE) technique cleaves chromosomal DNA at a single EcoRI site within a given gene or selected sequen...

the aim of present research was to locate genomic loci (qtl) on chromosome 2. itis associated with the carcass traits in japanese quail. the f1 population was created by using reciprocal crosses between two strains of white birds (layer) and wild (broiler) birds. the f2 birds (422 birds) were derived by random mating of the f1 birds. all of the f2 birds were slaughtered at 35 days of age and re...

Background and Aim: Brain mapping is a study of the anatomy and function of the CNS (central nervous system). Brain mapping has many techniques and these techniques are permanently changing and updating. From the beginning, brain mapping was invasive and for brain mapping, electrical stimulation of the exposed brain was needed. However, nowadays brain mapping does not require electrical stimula...

RNA-seq has become increasingly popular in transcriptome profiling. One of the major challenges in RNA-seq data analysis is the accurate mapping of junction reads to their genomic origins. To detect splicing sites in short reads, many RNA-seq aligners use reference transcriptome to inform placement of junction reads. However, no systematic evaluation has been performed to assess or quantify the...