نتایج جستجو برای: genome size
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Very low levels of genetic diversity have been reported in vertebrates with large genomes, notably salamanders and lungfish [1-3]. Interpreting differences in heterozygosity, which reflects genetic diversity in a population, is complicated because levels of heterozygosity vary widely between conspecific populations, and correlate with many different physiological and demographic variables such ...
The predominantly aquatic order Alismatales, which includes approximately 4,500 species within Araceae, Tofieldiaceae, and the core alismatid families, is a key group in investigating the origin and early diversification of monocots. Despite their importance, phylogenetic ambiguity regarding the root of the Alismatales tree precludes answering questions about the early evolution of the order. H...
Next-generation whole genome sequencing of microbes demands rapid, robust, and scalable library construction workflows, capable of generating high-quality sequence data across a wide range of genome sizes, complexities and genomic GC content. In this Application Note, we describe a streamlined library preparation method that results in minimal bias, high uniform coverage, and facilitates de nov...
Stripe rust of wheat, caused by Puccinia striiformis f. sp. tritici, is one of the important diseases of wheat. We used NGS technologies to generate a draft genome sequence of two highly virulent (46S 119 and 31) and a least virulent (K) pathotypes of P. striiformis from the Indian subcontinent. We generated ~24,000-32,000 sequence contigs (N50;7.4-9.2 kb), which accounted for ~86X-105X sequenc...
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