Hymenolepis nana is a common parasite of rodents as well as human intestine. This parasite has beenreported from all over the world, including Iran. The infection rate has been reported up to 40% in someareas. The infection has various clinical manifestations. The parasite could establish severe hyperinfection inpatients with immune deficiency. Regarding the rodents as hosts of the parasite, th...

Gene polymorphism refers to a variation in DNA sequence that occurs population with frequency of 1% or higher. Polymorphism may be single nucleotide (SNP) some repetitive sequences (length polymorphism). Several methods can used analyze polymorphism, included Polymerase Chain Reaction (PCR), sequencing from the conventional method more sophisticated such as Next Generation Sequencing (NGS), flu...

A complete hydatidiform mole (CHM) is a conceptus with only sperm-derived chromosomes. Here, we report on CHM genomic DNA identical to that of the paternal somatic cells. The developed in woman who had undergone intrauterine implantation blastocyst obtained through vitro injection presumed round spermatid into one her oocytes. was genetically peripheral white cells husband and contained no mate...

This Application Note demonstrates the use and application of the Agilent Genomic DNA ScreenTape assay in conjunction with the Agilent 2200 TapeStation system for easy sizing, quantification and quality assessment of genomic DNA samples. Intact, high quality genomic DNA in combination with accurate sample quantification is required for numerous applications, particularly array comparative genom...

Genomic selection (GS) integrates DNA marker and trait data to develop a model that enables prediction of performance (genomic-estimated breeding values; GEBVs) based on genotype alone. GS has been shown improve the efficiency effectiveness programmes, especially for complex traits such dry matter yield (DMY). DMY were generated from training population 200 white clover half-sibling (HS) famili...

The aim of the present study was to develop a simple, rapid and inexpensive protocol of ultrapure bacterial genomic DNA extraction suitable for use in molecular methodology. This communication describes a protocol which uses superparamagnetic bare nanoparticles for isolation and purification of genomic DNA from overnight culture of Escherichia coli XL1 Blue strain. A comparison is also made wit...

Genetic variation and inheritance of randomly amplified polymorphic DNA (RAPD) markers in female grass carp (Ctenopharyngodon idella) and male bighead carp (Hypophthalmichthys nobilis) and their F1 offspring hybrid have been studied. For this purpose, genomic DNA was extracted from muscle tissue according to phenol-chloroform method, and six decamer primers were used for amplifying polymorphic ...

Total genomic DNA from Bursaphelenchus xylophilus pathotypes MPSy-1 and VPSt-1 and from B. mucronatus was digested with restriction endonucleases. DNA fragments were electrophoretically separated, Southern blotted to nitrocellulose, and hybridized to genomic DNA from one of the isolates. The resulting hybridization patterns indicate genomic differences in repetitive DNA sequences among these po...

The selection of a DNA extraction method is a critical step when subsequent analysis depends on the DNA quality and quantity. Unlike mammals, for which several capable DNA extraction methods have been developed, for molluscs the availability of optimized genomic DNA extraction protocols is clearly insufficient. Several aspects such as animal physiology, the type (e.g., adductor muscle or gills)...

Single base pair differences between otherwise identical DNA molecules can result in altered melting behavior detectable by denaturing gradient gel electrophoresis. We have developed a simplified procedure for using denaturing gradient gel electrophoresis to detect base pair changes in genomic DNA. Genomic DNA is digested with restriction enzymes and hybridized in solution to labeled single-str...