Reference strains of infectious pancreatic necrosis virus resembling the 10 recognized serotypes and local isolates of aquabirnaviruses isolated in northwestern Spain from reservoirs (mollusks) and from asymptomatic and carrier cultured fish were genotyped by restriction fragment length polymorphism (RFLP) and nucleic acid sequence analyses. The RFLP analysis yielded seven genogroups, each of w...

Background Congenital toxoplasmosis is an important cause of spontaneous abortion worldwide. However, there is limited information on detection and genotypic characterization of T. gondii in women with recurrent spontaneous abortion (RSA). The aim of this study is molecular detection and genotypic characterization of T. gondii in formalin-fixed, paraffin-embedded fetoplacental tissues (FFPTs) o...

Using Mahalanobis D2statistics, the level of genetic diversity among soybean genotypes based on 12 agro-morphological traits was investigated in this study. A total 100 were grouped into six clusters. Cluster I had maximum number (53 genotypes), followed by cluster II (37 III (6 clusters IV and V (1 genotype each), VI (2 genotypes). Clusters greatest inter-cluster distance (117618.10). Intra-cl...

OBJECTIVES To examine the genotype:phenotype association in children compared with their parents. METHODS Variations at 4 key gene loci, namely lipoprotein lipase (LPL S447X), hepatic lipase (HL -480C>T), cholesteryl ester transfer protein (CETP TaqIB), and apolipoprotein CIII (APOC3 -455T>C and -482C>T), were examined in children (n = 495) and their parents (n = 353) in the Columbia Universi...

DNA fingerprinting of Candida dubliniensis isolates using the species-specific probe Cd25 previously showed that this species consists of two distinct groups, termed Cd25 group I and Cd25 group II. The present study investigated the population structure of 30 C. dubliniensis oral isolates from Saudi Arabia and Egypt using Cd25 fingerprinting and rRNA gene internal transcribed spacer region-base...

BACKGROUND/AIMS Many candidate gene studies have revealed that polymorphisms of the 5'-flanking controlled SERT gene linked polymorphic region (5HTT-LPR) gene and G-protein β3 C825T gene might be associated with functional dyspepsia (FD) and irritable bowel syndrome (IBS). This study was performed to investigate polymorphisms of the 5HTT-LPR gene and G-protein β3 C825T gene in FD and IBS in Kor...

This study identified subgenic PCR amplimers from 18S rDNA that were (i) highly specific for the genus Acanthamoeba, (ii) obtainable from all known genotypes, and (iii) useful for identification of individual genotypes. A 423- to 551-bp Acanthamoeba-specific amplimer ASA.S1 obtained with primers JDP1 and JDP2 was the most reliable for purposes i and ii. A variable region within this amplimer al...

PURPOSE Elevated levels of human telomerase (hTERT) mRNA in tumors is a marker for poorer survival in patients with stage I non-small cell lung cancer (NSCLC). A functional variant of MNS16A-short tandem repeats in hTERT (S allele) is associated with higher expression levels of hTERT mRNA compared with the MNS16A-long (L) allele. It is unknown, however, whether or not the hTERT MNS16A variant g...

The study aimed to evaluate the genetic diversity of eight brinjal (Solanum melongena L.) genotypes including wild species and develop a molecular profile using 14 SSR primers. value similarity matrix ranged from 0.12–0.87. Dendrogram image is revealing relationship among brinjal. super cluster divided into two clusters A B at Jaccard’s coefficient 0.31. Cluster again divide sub A1 A2 0.45. con...

OBJECTIVE To determine the efficacy of 12 weeks therapy with conventional interferon and ribavirin in chronic hepatitis C genotype 2 and 3 naive patients. STUDY DESIGN A randomized clinical trial. PLACE AND DURATION OF STUDY Postgraduate Medical Institute, Lady Reading Hospital, Peshawar, from January 2005 to October 2006. METHODOLOGY Two hundred and twenty seven patients with chronic hep...